Expression of polyhydroxybutyric acid as a model for metabolic engineering of soybean seed coats

被引：7
|
作者：
Schnell, J. A. [1 ]
Treyvaud-Amiguet, V. [1 ]
Arnason, J. T. [1 ]
Johnson, D. A. [1 ]
机构：
[1] Univ Ottawa, Ottawa Carleton Inst Biol, Stn A, Ottawa, ON K1N 6N5, Canada
来源：
TRANSGENIC RESEARCH | 2012年 / 21卷 / 04期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Soybean seed coat; Metabolic engineering; Bioplastics; Polyhydroxybutyrate; POLY-BETA-HYDROXYBUTYRATE; ALCALIGENES-EUTROPHUS H16; TRANSGENIC PLANTS; BIOSYNTHETIC-PATHWAY; ESCHERICHIA-COLI; PEROXIDASE; GENE; KETOTHIOLASE; PROTEIN; CLONING;
D O I：
10.1007/s11248-011-9575-x
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The feasibility of genetically engineering soybean seed coats to divert metabolism towards the production of novel biochemicals was tested. The genes phbA, phbB, phbC from Ralstonia eutropha each under the control of the seed coat peroxidase promoter were introduced into soybean and the production of polyhydroxybutyrate (PHB) was assayed. The analysis of seed coats arising from 4 independent transformation events demonstrated that PHB was produced at a mean of 0.12% seed coat dried weight with individual values up to 0.36%. These values demonstrate that it is possible to metabolically engineer soybean seed coats.
引用
收藏
页码：895 / 899
页数：5
相关论文
共 50 条
  • [31] Cracks in the palisade cuticle of soybean seed coats correlate with their permeability to water
    Ma, FS
    Cholewa, E
    Mohamed, T
    Peterson, CA
    Gijzen, M
    ANNALS OF BOTANY, 2004, 94 (02) : 213 - 228
  • [32] EXCISED SEED COATS OF SOYBEAN - A SUBSTRATE FOR ISOLATING CERCOSPORA-KIKUCHII
    RICHARDS, TL
    ABNEY, TS
    PHYTOPATHOLOGY, 1981, 71 (02) : 251 - 251
  • [33] Improving Soybean Seed Performance with Natural Colorant-based Novel Seed-Coats
    Tripathi, Brijesh
    Pandey, Alka
    Bhatia, Rohit
    Walia, Suresh
    Yadav, Anil Kumar
    JOURNAL OF CROP IMPROVEMENT, 2015, 29 (03) : 301 - 318
  • [34] ENGINEERING OF SOYBEAN SEED STORAGE PROTEINS
    DICKINSON, CD
    FLOENER, LA
    EVANS, RP
    NIELSEN, NC
    FEDERATION PROCEEDINGS, 1987, 46 (06) : 2023 - 2023
  • [35] CONCENTRATIONS OF SUCROSE AND NITROGENOUS COMPOUNDS IN THE APOPLAST OF DEVELOPING SOYBEAN SEED COATS AND EMBRYOS
    HSU, FC
    BENNETT, AB
    SPANSWICK, RM
    PLANT PHYSIOLOGY, 1984, 75 (01) : 181 - 186
  • [36] Optimization of the removal of phenol by soybean seed coats using response surface methodology
    Rezvani, F.
    Hashemi-Najafabadi, S.
    Mousavi, S. M.
    Shojaosadati, S. A.
    Saharkhiz, S.
    WATER SCIENCE AND TECHNOLOGY, 2012, 66 (10) : 2229 - 2236
  • [37] cDNA-AFLP analyses between black and yellow soybean seed coats
    Mertz, L. M.
    Henning, F. A.
    Borsuk, S.
    Da Maia, L. C.
    Dellagostin, O. A.
    Peske, S. T.
    Zimmer, P. D.
    SEED SCIENCE AND TECHNOLOGY, 2010, 38 (01) : 88 - 95
  • [38] INFLUENCE OF ETCHED SEED COATS ON PHOMOPSIS INFECTION AND ELECTROLYTE LEAKAGE OF SOYBEAN SEEDS
    THOMISON, PR
    KULIK, MM
    MORRIS, DA
    JOURNAL OF SEED TECHNOLOGY, 1989, 13 (01): : 9 - 18
  • [39] SGlu2 gene expression in coats of soybean seeds
    Bahry, Carlos Andre
    Zimmer, Paulo Dejalma
    JOURNAL OF SEED SCIENCE, 2014, 36 (03) : 290 - 294
  • [40] Genome-Wide Association Mapping for Seed Weight in Soybean with Black Seed Coats and Green Cotyledons
    Jo, Hyun
    Lee, Ji Yun
    Lee, Jeong-Dong
    AGRONOMY-BASEL, 2022, 12 (02):
12345