Expression of polyhydroxybutyric acid as a model for metabolic engineering of soybean seed coats

被引:0
|
作者
J. A. Schnell
V. Treyvaud-Amiguet
J. T. Arnason
D. A. Johnson
机构
[1] University of Ottawa,Ottawa
[2] Canadian Food Inspection Agency,Carleton Institute of Biology
[3] Health Canada,Plant and Biotechnology Risk Assessment Unit
来源
Transgenic Research | 2012年 / 21卷
关键词
Soybean seed coat; Metabolic engineering; Bioplastics; Polyhydroxybutyrate;
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学科分类号
摘要
The feasibility of genetically engineering soybean seed coats to divert metabolism towards the production of novel biochemicals was tested. The genes phbA, phbB, phbC from Ralstonia eutropha each under the control of the seed coat peroxidase promoter were introduced into soybean and the production of polyhydroxybutyrate (PHB) was assayed. The analysis of seed coats arising from 4 independent transformation events demonstrated that PHB was produced at a mean of 0.12% seed coat dried weight with individual values up to 0.36%. These values demonstrate that it is possible to metabolically engineer soybean seed coats.
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页码:895 / 899
页数:4
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