A Gain-of-Function Mutation in Gating of Corynebacterium glutamicum NCgl1221 Causes Constitutive Glutamate Secretion

被引：22
|
作者：
Nakayama, Yoshitaka [1 ]
Yoshimura, Kenjiro [2 ]
Iida, Hidetoshi [1 ]
机构：
[1] Tokyo Gakugei Univ, Dept Biol, Koganei, Tokyo 184, Japan
[2] Univ Maryland, Dept Biol, College Pk, MD 20742 USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2012年 / 78卷 / 15期
基金：
日本学术振兴会;
关键词：
ESCHERICHIA-COLI MSCS; MECHANOSENSITIVE CHANNEL; ACID;
D O I：
10.1128/AEM.01310-12
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The A-to-V mutation at position 111 (A111V) in the mechanosensitive channel NCgl1221 (MscCG) causes constitutive glutamate secretion in Corynebacterium glutamicum. Patch clamp experiments revealed that NCgl1221 (A111V) had a significantly smaller gating threshold than the wild-type counterpart and displayed strong hysteresis, suggesting that the gain-of-function mutation in the gating of NCgl1221 leads to the oversecretion of glutamate.
引用
收藏
页码：5432 / 5434
页数：3
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