Genetically Encoded Copper-Free Click Chemistry

被引：235

作者：

Plass, Tilman ^{[1
,2
]}

Milles, Sigrid ^{[1
,2
]}

Koehler, Christine ^{[1
,2
]}

Schultz, Carsten ^{[1
,2
]}

Lemke, Edward A. ^{[1
,2
]}

机构：

[1] EMBL, Struct & Computat Biol Unit, D-69117 Heidelberg, Germany

[2] EMBL, Cell Biol & Biophys Unit, D-69117 Heidelberg, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 17期

关键词：

amino acids; click chemistry; gene expression; proteins; strained molecules; FLUORESCENT AMINO-ACID; LIVING CELLS; LIVE CELLS; RECOMBINANT PROTEINS; MAMMALIAN-CELLS; CYCLOADDITION; AZIDE; CODE; LYSINE;

D O I：

10.1002/anie.201008178

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A bright click of life: Strained alkynes are genetically encoded into E. coli by use of an engineered pyrrolysine amber suppressor tRNA/synthetase pair from Methanosarcina mazei. As a result cyclooctynyl lysine derivatives were incorporated site-specifically in proteins allowing efficient labeling with fluorogenic azide-bearing dyes by means of a click reaction under physiological conditions in vitro and in vivo (see scheme). © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：3878 / 3881

页数：4

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