Genetically Encoded Copper-Free Click Chemistry

被引：235
|
作者：
Plass, Tilman [1 ,2 ]
Milles, Sigrid [1 ,2 ]
Koehler, Christine [1 ,2 ]
Schultz, Carsten [1 ,2 ]
Lemke, Edward A. [1 ,2 ]
机构：
[1] EMBL, Struct & Computat Biol Unit, D-69117 Heidelberg, Germany
[2] EMBL, Cell Biol & Biophys Unit, D-69117 Heidelberg, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 17期
关键词：
amino acids; click chemistry; gene expression; proteins; strained molecules; FLUORESCENT AMINO-ACID; LIVING CELLS; LIVE CELLS; RECOMBINANT PROTEINS; MAMMALIAN-CELLS; CYCLOADDITION; AZIDE; CODE; LYSINE;
D O I：
10.1002/anie.201008178
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A bright click of life: Strained alkynes are genetically encoded into E. coli by use of an engineered pyrrolysine amber suppressor tRNA/synthetase pair from Methanosarcina mazei. As a result cyclooctynyl lysine derivatives were incorporated site-specifically in proteins allowing efficient labeling with fluorogenic azide-bearing dyes by means of a click reaction under physiological conditions in vitro and in vivo (see scheme). © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：3878 / 3881
页数：4
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