β2-Adrenergic receptors and G-protein-coupled receptor kinase 2 in rabbit pleural mesothelium

被引：2
|
作者：
Sironi, Chiara [1 ]
Bodega, Francesca [1 ]
Armilli, Marta [1 ]
Porta, Cristina [1 ]
Zocchi, Luciano [1 ]
Agostoni, Emilio [1 ]
机构：
[1] Univ Milan, Dipartimento Fisiol Umana, I-20133 Milan, Italy
来源：
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY | 2010年 / 173卷 / 02期
关键词：
beta 2-Adrenergic receptors; G-protein-coupled receptor kinase 2; Pleural liquid absorption; Pleural liquid dilution; Pleural lubrication; Rabbit mesothelium; LUBRICATION; TRANSPORT; EXPRESSION;
D O I：
10.1016/j.resp.2010.07.004
中图分类号：
Q4 [生理学];
学科分类号：
071003 ;
摘要：
Former studies on net rate of liquid absorption from small Ringer or 1% albumin-Ringer hydrothoraces in rabbits indicated that Na+ transport and solute-coupled liquid absorption by mesothelium is increased by pleural liquid dilution, and stimulation of beta 2-adrenoreceptors(beta 2AR) In this research we tried to provide molecular evidence for beta 2AR in visceral and parietal mesothelium of rabbit pleura. Moreover, because prolonged stimulation of beta 2AR may lead to desensitization mediated by G-protein-coupled receptor kinase 2 (GRK2), we also checked whether GRK2 is expressed in pleural mesothelium To this end we performed immunoblot assays on total protein extracts from scraped visceral and parietal mesothelium, and from cultured pleural mesothelial cells of rabbits All three samples showed beta 2AR and GRK2 specific bands (C) 2010 Elsevier B V All rights reserved
引用
收藏
页码：189 / 191
页数：3
相关论文
共 50 条
  • [31] Modulation of histamine H2 receptor signalling by G-protein-coupled receptor kinase 2 and 3
    Rodriguez-Pena, MS
    Timmerman, H
    Leurs, R
    BRITISH JOURNAL OF PHARMACOLOGY, 2000, 131 (08) : 1707 - 1715
  • [32] G-protein-coupled receptor kinase expression in hypertension
    Gros, R
    Tan, CM
    Chorazyczewski, J
    Kelvin, DJ
    Benovic, JL
    Feldman, RD
    CLINICAL PHARMACOLOGY & THERAPEUTICS, 1999, 65 (05) : 545 - 551
  • [33] Structure of a β1-adrenergic G-protein-coupled receptor
    Tony Warne
    Maria J. Serrano-Vega
    Jillian G. Baker
    Rouslan Moukhametzianov
    Patricia C. Edwards
    Richard Henderson
    Andrew G. W. Leslie
    Christopher G. Tate
    Gebhard F. X. Schertler
    Nature, 2008, 454 : 486 - 491
  • [34] Structure of a β1-adrenergic G-protein-coupled receptor
    Warne, Tony
    Serrano-Vega, Maria J.
    Baker, Jillian G.
    Moukhametzianov, Rouslan
    Edwards, Patricia C.
    Henderson, Richard
    Leslie, Andrew G. W.
    Tate, Christopher G.
    Schertler, Gebhard F. X.
    NATURE, 2008, 454 (7203) : 486 - 491
  • [35] G-protein-coupled receptor kinase 2 in pancreatic cancer: clinicopathologic and prognostic significance
    Zhou, Li
    Wang, Meng-Yi
    Liang, Zhi-Yong
    Zhou, Wei-Xun
    You, Lei
    Pan, Bo-Ju
    Liao, Quan
    Zhao, Yu-Pei
    HUMAN PATHOLOGY, 2016, 56 : 171 - 177
  • [36] Molecular dynamics of a biophysical model for β2-adrenergic and G protein-coupled receptor activation
    Rubenstein, Lester A.
    Zauhar, Randy J.
    Lanzara, Richard G.
    JOURNAL OF MOLECULAR GRAPHICS & MODELLING, 2006, 25 (04): : 396 - 409
  • [37] An RNA molecule that specifically inhibits G-protein-coupled receptor kinase 2 in vitro
    Mayer, Guenter
    Wulffen, Bernhard
    Huber, Christian
    Brockmann, Joerg
    Flicke, Birgit
    Neumann, Lars
    Hafenbradl, Doris
    Klebl, Bert M.
    Lohse, Martin J.
    Krasel, Cornelius
    Blind, Michael
    RNA, 2008, 14 (03) : 524 - 534
  • [38] G-protein-coupled receptor kinase 2 and hypertension: Molecular insights and pathophysiological mechanisms
    Santulli G.
    Trimarco B.
    Iaccarino G.
    High Blood Pressure & Cardiovascular Prevention, 2013, 20 (1) : 5 - 12
  • [39] A CLONING STRATEGY FOR G-PROTEIN-COUPLED HORMONE RECEPTORS - THE OVINE BETA(1)-ADRENERGIC RECEPTOR
    PADBURY, JF
    TSENG, YT
    WASCHEK, JA
    REPRODUCTION FERTILITY AND DEVELOPMENT, 1995, 7 (03) : 521 - 525
  • [40] β2-Adrenergic receptors potentiate glucocorticoid receptor transactivation via G protein βγ-subunits and the phosphoinositide 3-kinase pathway
    Schmidt, P
    Holsboer, F
    Spengler, D
    MOLECULAR ENDOCRINOLOGY, 2001, 15 (04) : 553 - 564
12345