β2-Adrenergic receptors and G-protein-coupled receptor kinase 2 in rabbit pleural mesothelium

被引：2

作者：

Sironi, Chiara ^{[1
]}

Bodega, Francesca ^{[1
]}

Armilli, Marta ^{[1
]}

Porta, Cristina ^{[1
]}

Zocchi, Luciano ^{[1
]}

Agostoni, Emilio ^{[1
]}

机构：

[1] Univ Milan, Dipartimento Fisiol Umana, I-20133 Milan, Italy

来源：

RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY | 2010年 / 173卷 / 02期

关键词：

beta 2-Adrenergic receptors; G-protein-coupled receptor kinase 2; Pleural liquid absorption; Pleural liquid dilution; Pleural lubrication; Rabbit mesothelium; LUBRICATION; TRANSPORT; EXPRESSION;

D O I：

10.1016/j.resp.2010.07.004

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Former studies on net rate of liquid absorption from small Ringer or 1% albumin-Ringer hydrothoraces in rabbits indicated that Na+ transport and solute-coupled liquid absorption by mesothelium is increased by pleural liquid dilution, and stimulation of beta 2-adrenoreceptors(beta 2AR) In this research we tried to provide molecular evidence for beta 2AR in visceral and parietal mesothelium of rabbit pleura. Moreover, because prolonged stimulation of beta 2AR may lead to desensitization mediated by G-protein-coupled receptor kinase 2 (GRK2), we also checked whether GRK2 is expressed in pleural mesothelium To this end we performed immunoblot assays on total protein extracts from scraped visceral and parietal mesothelium, and from cultured pleural mesothelial cells of rabbits All three samples showed beta 2AR and GRK2 specific bands (C) 2010 Elsevier B V All rights reserved

引用

页码：189 / 191

页数：3

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