Replacing a reservoir solution with desiccant in vapor diffusion protein crystallization screening

被引：9

作者：

Lu, Qin-Qin ^{[1
]}

Yin, Da-Chuan ^{[1
]}

Chen, Rui-Qing ^{[1
]}

Xie, Si-Xiao ^{[1
]}

Liu, Yong-Ming ^{[1
]}

Zhang, Xian-Fang ^{[1
]}

Zhu, Li ^{[1
]}

Liu, Zheng-Tang ^{[2
]}

Shang, Peng ^{[1
]}

机构：

[1] NW Polytech Univ, Inst Special Environm Biophys, Key Lab Space Bioscience & Space Biotechnol, Sch Life Sci, Xian 710072, Shaanxi, Peoples R China

[2] NW Polytech Univ, Sch Mat Sci & Engn, Xian 710072, Shaanxi, Peoples R China

来源：

JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2010年 / 43卷

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

protein crystallization; vapor diffusion; crystallization screening; desiccants; MICROBATCH METHOD; EVAPORATION;

D O I：

10.1107/S0021889810030803

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This paper presents a modification to the conventional vapor diffusion (hanging- or sitting-drop) technique for protein crystallization screening. In this modified method, the reservoir solution is replaced with a desiccant to allow for a larger range of protein solution concentrations, thereby providing more opportunities for crystal formation. This method was tested in both reproducibility and screening studies, and the results showed that it significantly improves the efficiency and reduces the cost of protein crystallization screens.

引用

页码：1021 / 1026

页数：6

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