Replacing a reservoir solution with desiccant in vapor diffusion protein crystallization screening

被引：9

作者：

Lu, Qin-Qin ^{[1
]}

Yin, Da-Chuan ^{[1
]}

Chen, Rui-Qing ^{[1
]}

Xie, Si-Xiao ^{[1
]}

Liu, Yong-Ming ^{[1
]}

Zhang, Xian-Fang ^{[1
]}

Zhu, Li ^{[1
]}

Liu, Zheng-Tang ^{[2
]}

Shang, Peng ^{[1
]}

机构：

[1] NW Polytech Univ, Inst Special Environm Biophys, Key Lab Space Bioscience & Space Biotechnol, Sch Life Sci, Xian 710072, Shaanxi, Peoples R China

[2] NW Polytech Univ, Sch Mat Sci & Engn, Xian 710072, Shaanxi, Peoples R China

来源：

JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2010年 / 43卷

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

protein crystallization; vapor diffusion; crystallization screening; desiccants; MICROBATCH METHOD; EVAPORATION;

D O I：

10.1107/S0021889810030803

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This paper presents a modification to the conventional vapor diffusion (hanging- or sitting-drop) technique for protein crystallization screening. In this modified method, the reservoir solution is replaced with a desiccant to allow for a larger range of protein solution concentrations, thereby providing more opportunities for crystal formation. This method was tested in both reproducibility and screening studies, and the results showed that it significantly improves the efficiency and reduces the cost of protein crystallization screens.

引用

页码：1021 / 1026

页数：6

共 50 条

[11] Application of a stirring method to micro-scale and vapor diffusion protein crystallization
Adachi, H
Takano, K
Yoshimura, M
Mori, Y
Sasaki, T
JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS, 2003, 42 (3B): : L314 - L315
[12] Application of a stirring method to micro-scale and vapor diffusion protein crystallization
Adachi, Hiroaki
Takano, Kazufumi
Yoshimura, Masashi
Mori, Yusuke
Sasaki, Takatomo
Adachi, H., 1600, Japan Society of Applied Physics (42):
[13] Macromolecular crystallization microarrayed chip for super saturated vapor and liquid-liquid diffusion screening
Akioka, K
Inoue, S
Sanjoh, A
Tsukihara, T
Gorti, S
MICRO TOTAL ANALYSIS SYSTEMS 2000, PROCEEDINGS, 2000, : 607 - 610
[14] Crystallization of a human Bence-Jones protein in microgravity using vapor diffusion in capillaries
Alvarado, UR
DeWitt, CR
Shultz, BB
Ramsland, PA
Edmundson, AB
JOURNAL OF CRYSTAL GROWTH, 2001, 223 (03) : 407 - 414
[15] Efficient Screening Methodology for Protein Crystallization Based on the Counter-Diffusion Technique
Gonzalez-Ramirez, Luis A.
Ruiz-Martinez, Carlos R.
Estremera-Andujar, Rafael A.
Nieves-Marrero, Carlos A.
Garcia-Caballero, Alfonso
Gavira, Jose A.
Lopez-Garriga, Juan
Garcia-Ruiz, Juan M.
CRYSTAL GROWTH & DESIGN, 2017, 17 (12) : 6780 - 6786
[16] 3D-Printed High-Density Droplet Array Chip for Miniaturized Protein Crystallization Screening under Vapor Diffusion Mode
Liang, Yi-Ran
Zhu, Li-Na
Gao, Jie
Zhao, Hong-Xia
Zhu, Ying
Ye, Sheng
Fang, Qun
ACS APPLIED MATERIALS & INTERFACES, 2017, 9 (13) : 11837 - 11845
[17] Kinetics of pH changes in the vapor diffusion method of protein crystallization using ammonium sulfate as the precipitant
Rodeau, J.-L., 1600, (24):
[18] Two-Liquid Hanging-Drop Vapor-Diffusion Technique of Protein Crystallization
Adachi, H. (adachi@pwr.eng.osaka-u.ac.jp), 1600, Japan Society of Applied Physics (43):
[19] Toward a comparative study of protein crystallization in microfluidic chambers using vapor diffusion and batch techniques
Lounaci, M.
Rigolet, P.
Casquillas, G. Velve
Huang, H. W.
Chen, Y.
MICROELECTRONIC ENGINEERING, 2006, 83 (4-9) : 1673 - 1676
[20] Two-liquid hanging-drop vapor-diffusion technique of protein crystallization
Adachi, H
Matsumura, H
Takano, K
Niino, A
Inoue, T
Mori, Y
Sasaki, T
JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS, 2004, 43 (1A-B): : L79 - L81

← 1 2 3 4 5 →