Multiplex allele-specific PCR combined with PCR-RFLP analysis for rapid detection of gyrA gene fluoroquinolone resistance mutations in Mycobacterium tuberculosis

被引：9

作者：

Zhao, Li-li ^{[1
]}

Xia, Qiang ^{[1
,2
]}

Lin, Nan ^{[3
,4
]}

Liu, Zhi-guang ^{[1
]}

Zhao, Xiu-Qin ^{[1
]}

Wan, Kang-lin ^{[1
]}

机构：

[1] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China

[2] Zhejiang TCM & WM Hosp, Zhejiang Prevent & Treatment Ctr TB, Hangzhou 310003, Zhejiang, Peoples R China

[3] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

[4] Fujian Agr & Forestry Univ, Minist Educ, Key Lab Biopesticide & Chem Biol, Coll Life Sci, Fuzhou 350002, Peoples R China

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 88卷 / 01期

关键词：

Mycobacterium tuberculosis; Multiplex allele-specific PCR; Restriction fragment length polymorphism; Fluoroquinolone; SYSTEM;

D O I：

10.1016/j.mimet.2011.10.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A combined use of MAS-PCR (multiplex allele-specific PCR) and PCR-RFLP (KR restriction fragment length polymorphism), was established to detect mutations in codons 90,91 and 94 of the gyrA gene in Mycobacterium tuberculosis (M. tuberculosis). With conventional phenotypic drug susceptibility testing as a reference standard, the sensitivity, specificity and accuracy of the modified method for gyrA gene mutation detection were 70.8%, 100% and 84.8% respectively. (C) 2011 Published by Elsevier B.V.

引用

页码：175 / 178

页数：4

共 50 条

[31] Detection of isoniazid-resistant Mycobacterium tuberculosis strains by a multiplex allele-specific PCR assay targeting katG codon 315 variation
Mokrousov, I
Otten, T
Filipenko, M
Vyazovaya, A
Chrapov, E
Limeschenko, E
Steklova, L
Vyshnevskiy, B
Narvskaya, O
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (07) : 2509 - 2512
[32] Rapid detection of multidrug-resistant Mycobacterium tuberculosis by multiplex allele-specific polymerase chain reaction
Imperiale, B. R.
Cataldi, A. A.
Morcillo, N. S.
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE, 2011, 15 (04) : 496 - 501
[33] Allele-specific rpoB PCR assays for detection of rifampin-resistant Mycobacterium tuberculosis in sputum smears
Mokrousov, I
Otten, T
Vyshnevskiy, B
Narvskaya, O
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (07) : 2231 - 2235
[34] Combination of enzyme analysis, allele-specific PCR and sequencing to detect mutations in the GALT gene
Calderon, F. R. O.
Nelson, L.
Dobrowolski, P.
Sinitsyna, I.
Phansalkar, A.
Longo, N.
Pasquali, M.
Mao, R.
JOURNAL OF INHERITED METABOLIC DISEASE, 2007, 30 (05) : 818 - 818
[35] Detection of Mycobacterium avium & M-tuberculosis from human sputum cultures by PCR-RFLP analysis of hsp65 gene & pncA PCR
Bannalikar, AS
Verma, R
INDIAN JOURNAL OF MEDICAL RESEARCH, 2006, 123 (02) : 165 - 172
[36] Detection of KatG Mutation in MDR Mycobacterium Tuberculosis Isolates by PCR-RFLP and DNA Sequencing
Ilyas, Muhammad
Niaz, Falak
Ishaq, Rafaqat
Rafiullah
Khanum, Azra
BANGLADESH JOURNAL OF MEDICAL SCIENCE, 2023, 22 (04): : 804 - 808
[37] Detection of rifampicin resistance of Mycobacterium tuberculosis using multiplex allele specific polymerase chain reaction (MAS-PCR) in Pakistan
Ullah, Irfan
Ahmad, Waqas
Shah, Aamer Ali
Shahzada, Amir
Tahir, Zarfishan
Qazi, Obaidullah
Hasan, Fariha
Ayub, Najma
Badar, Muhammad
Butt, Zahid Ahmad
Basit, Sulman
INFECTION GENETICS AND EVOLUTION, 2019, 71 : 42 - 46
[38] A combined allele-specific PCR and RFLP assay to detect the 35delG mutation in the connexin 26 gene
Baris, I
Koksal, V
Etlik, O
GENETIC TESTING, 2004, 8 (04): : 384 - 386
[39] Rapid Resistance Detection of Amaranthus retroflexus to Fomesafen via Kompetitive Allele-Specific PCR (KASP)
Zhang, Zhanzhan
Ban, Yaxin
Wei, Jianguo
Wu, Qun
Dong, Liyao
Feng, Zhike
PLANTS-BASEL, 2025, 14 (04):
[40] A simple and rapid cis-AB genotyping by multiplex allele-specific PCR.
Suh, SP
Cho, D
Kee, S
Shin, M
Shin, J
Ryang, D
CLINICAL CHEMISTRY, 2004, 50 (06) : A176 - A176

← 1 2 3 4 5 →