Multiplex allele-specific PCR combined with PCR-RFLP analysis for rapid detection of gyrA gene fluoroquinolone resistance mutations in Mycobacterium tuberculosis

被引：9

作者：

Zhao, Li-li ^{[1
]}

Xia, Qiang ^{[1
,2
]}

Lin, Nan ^{[3
,4
]}

Liu, Zhi-guang ^{[1
]}

Zhao, Xiu-Qin ^{[1
]}

Wan, Kang-lin ^{[1
]}

机构：

[1] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China

[2] Zhejiang TCM & WM Hosp, Zhejiang Prevent & Treatment Ctr TB, Hangzhou 310003, Zhejiang, Peoples R China

[3] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

[4] Fujian Agr & Forestry Univ, Minist Educ, Key Lab Biopesticide & Chem Biol, Coll Life Sci, Fuzhou 350002, Peoples R China

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 88卷 / 01期

关键词：

Mycobacterium tuberculosis; Multiplex allele-specific PCR; Restriction fragment length polymorphism; Fluoroquinolone; SYSTEM;

D O I：

10.1016/j.mimet.2011.10.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A combined use of MAS-PCR (multiplex allele-specific PCR) and PCR-RFLP (KR restriction fragment length polymorphism), was established to detect mutations in codons 90,91 and 94 of the gyrA gene in Mycobacterium tuberculosis (M. tuberculosis). With conventional phenotypic drug susceptibility testing as a reference standard, the sensitivity, specificity and accuracy of the modified method for gyrA gene mutation detection were 70.8%, 100% and 84.8% respectively. (C) 2011 Published by Elsevier B.V.

引用

页码：175 / 178

页数：4

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