3D structure of the influenza virus polymerase complex:: Localization of subunit domains

被引：97

作者：

Area, E ^{[1
]}

Martín-Benito, J ^{[1
]}

Gastaminza, P ^{[1
]}

Torreira, E ^{[1
]}

Valpuesta, JM ^{[1
]}

Carrascosa, JL ^{[1
]}

Ortín, J ^{[1
]}

机构：

[1] Ctr Nacl Biotecnol, E-28049 Madrid, Spain

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2004年 / 101卷 / 01期

关键词：

D O I：

10.1073/pnas.0307127101

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The 3D structure of the influenza virus polymerase complex was determined by electron microscopy and image processing of recombinant ribonucleoproteins (RNPs). The RNPs were generated by in vivo amplification using cDNAs of the three polymerase subunits, the nucleoprotein, and a model virus-associated RNA containing 248 nt. The polymerase structure obtained is very compact, with no apparent boundaries among subunits. The position of specific regions of the PB1, PB2, and PA subunits was determined by 3D reconstruction of either RNP-mAb complexes or tagged RNPs. This structural model is available for the polymerase of a negative-stranded RNA virus and provides a general delineation of the complex and its interaction with the template-associated nucleoprotein monomers in the RNP.

引用

页码：308 / 313

页数：6

共 50 条

[41] The interaction of influenza A virus RNA polymerase PA subunit with the human β-actin protein
Gelmez, Nazife
Caglayan, Elif
Turan, Kadir
ACTA VIROLOGICA, 2024, 67
[42] Involvement of influenza virus PA subunit in assembly of functional RNA polymerase complexes
Kawaguchi, A
Naito, T
Nagata, K
JOURNAL OF VIROLOGY, 2005, 79 (02) : 732 - 744
[43] The structure of invadopodia in a complex 3D environment
Tolde, Ondrej
Roesel, Daniel
Vesely, Pavel
Folk, Petr
Brabek, Jan
EUROPEAN JOURNAL OF CELL BIOLOGY, 2010, 89 (09) : 674 - 680
[44] Structure of the Nipah virus polymerase phosphoprotein complex
Yang, Ge
Wang, Dong
Liu, Bin
NATURE COMMUNICATIONS, 2024, 15 (01)
[45] Structure of the Respiratory Syncytial Virus Polymerase Complex
Gilman, Morgan S. A.
Liu, Cheng
Fung, Amy
Behera, Ishani
Jordan, Paul
Rigaux, Peter
Ysebaert, Nina
Tcherniuk, Sergey
Sourimant, Julien
Eleouet, Jean-Francois
Sutto-Ortiz, Priscila
Decroly, Etienne
Roymans, Dirk
Jin, Zhinan
McLellan, Jason S.
CELL, 2019, 179 (01) : 193 - +
[46] Structure and nuclear import function of the C-terminal domain of influenza virus polymerase PB2 subunit
Tarendeau, Franck
Boudet, Julien
Guilligay, Delphine
Mas, Philippe J.
Bougault, Catherine M.
Boulo, Sebastien
Baudin, Florence
Ruigrok, Rob W. H.
Daigle, Nathalie
Ellenberg, Jan
Cusack, Stephen
Simorre, Jean-Pierre
Hart, Darren J.
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2007, 14 (03) : 229 - 233
[47] Structure and nuclear import function of the C-terminal domain of influenza virus polymerase PB2 subunit
Franck Tarendeau
Julien Boudet
Delphine Guilligay
Philippe J Mas
Catherine M Bougault
Sébastien Boulo
Florence Baudin
Rob W H Ruigrok
Nathalie Daigle
Jan Ellenberg
Stephen Cusack
Jean-Pierre Simorre
Darren J Hart
Nature Structural & Molecular Biology, 2007, 14 : 229 - 233
[48] Solution structure of Domains IVa and V of the τ subunit of Escherichia coli DNA polymerase III and interaction with the α subunit
Su, Xun-Cheng
Jergic, Slobodan
Keniry, Max A.
Dixon, Nicholas E.
Otting, Gottfried
NUCLEIC ACIDS RESEARCH, 2007, 35 (09) : 2825 - 2832
[49] CHARACTERIZATION OF SUBUNIT STRUCTURE OF RIBONUCLEIC ACID GENOME OF INFLUENZA VIRUS
LEWANDOWSKI, LJ
CONTENT, J
LEPPLA, SH
JOURNAL OF VIROLOGY, 1971, 8 (05) : 701 - +
[50] Crystal Structure of the Vaccinia Virus DNA Polymerase Holoenzyme Subunit D4 in Complex with the A20 N-Terminal Domain
Contesto-Richefeu, Celine
Tarbouriech, Nicolas
Brazzolotto, Xavier
Betzi, Stephane
Morelli, Xavier
Burmeister, Wim P.
Iseni, Frederic
PLOS PATHOGENS, 2014, 10 (03)

← 1 2 3 4 5 →