3D structure of the influenza virus polymerase complex:: Localization of subunit domains

被引：97
|
作者：
Area, E [1 ]
Martín-Benito, J [1 ]
Gastaminza, P [1 ]
Torreira, E [1 ]
Valpuesta, JM [1 ]
Carrascosa, JL [1 ]
Ortín, J [1 ]
机构：
[1] Ctr Nacl Biotecnol, E-28049 Madrid, Spain
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2004年 / 101卷 / 01期
关键词：
D O I：
10.1073/pnas.0307127101
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The 3D structure of the influenza virus polymerase complex was determined by electron microscopy and image processing of recombinant ribonucleoproteins (RNPs). The RNPs were generated by in vivo amplification using cDNAs of the three polymerase subunits, the nucleoprotein, and a model virus-associated RNA containing 248 nt. The polymerase structure obtained is very compact, with no apparent boundaries among subunits. The position of specific regions of the PB1, PB2, and PA subunits was determined by 3D reconstruction of either RNP-mAb complexes or tagged RNPs. This structural model is available for the polymerase of a negative-stranded RNA virus and provides a general delineation of the complex and its interaction with the template-associated nucleoprotein monomers in the RNP.
引用
收藏
页码：308 / 313
页数：6
相关论文
共 50 条
  • [21] Fine mapping of the subunit binding sites of influenza virus RNA polymerase
    Ohtsu, Y
    Honda, Y
    Toyoda, T
    OPTIONS FOR THE CONTROL OF INFLUENZA IV, 2001, 1219 : 463 - 469
  • [22] Inhibition of herpesvirus and influenza virus replication by blocking polymerase subunit interactions
    Palu, Giorgio
    Loregian, Arianna
    ANTIVIRAL RESEARCH, 2013, 99 (03) : 318 - 327
  • [23] Defective assembly of influenza A virus due to a mutation in the polymerase subunit PA
    Regan, JF
    Liang, YY
    Parslow, TG
    JOURNAL OF VIROLOGY, 2006, 80 (01) : 252 - 261
  • [24] Fine mapping of the subunit binding sites of influenza virus RNA polymerase
    Ohtsu, Y
    Honda, Y
    Sakata, Y
    Kato, H
    Toyoda, T
    MICROBIOLOGY AND IMMUNOLOGY, 2002, 46 (03) : 167 - 175
  • [25] The structural basis for an essential subunit interaction in influenza virus RNA polymerase
    Obayashi, Eiji
    Yoshida, Hisashi
    Kawai, Fumihiro
    Shibayama, Naoya
    Kawaguchi, Atsushi
    Nagata, Kyosuke
    Tame, Jeremy R. H.
    Park, Sam-Yong
    NATURE, 2008, 454 (7208) : 1127 - U57
  • [26] Structure-virus relationships of the influenza virus RNA polymerase
    Honda, A
    Ishihama, A
    OPTIONS FOR THE CONTROL OF INFLUENZA V, 2004, 1263 : 21 - 24
  • [27] Structure of the θ subunit of Escherichia coli DNA polymerase III in complex with the ε subunit
    Keniry, Max A.
    Park, Ah Young
    Owen, Elisabeth A.
    Hamdan, Samir M.
    Pintacuda, Guido
    Otting, Gottfried
    Dixon, Nicholas E.
    JOURNAL OF BACTERIOLOGY, 2006, 188 (12) : 4464 - 4473
  • [28] PA subunit from influenza virus polymerase complex interacts with a cellular protein with homology to a family of transcriptional activators
    Huarte, M
    Sanz-Ezquerro, JJ
    Roncal, F
    Ortín, J
    Nieto, A
    JOURNAL OF VIROLOGY, 2001, 75 (18) : 8597 - 8604
  • [29] Structure of the Ebola virus polymerase complex
    Bin Yuan
    Qi Peng
    Jinlong Cheng
    Min Wang
    Jin Zhong
    Jianxun Qi
    George F. Gao
    Yi Shi
    Nature, 2022, 610 : 394 - 401
  • [30] Structure of the Ebola virus polymerase complex
    Yuan, Bin
    Peng, Qi
    Cheng, Jinlong
    Wang, Min
    Zhong, Jin
    Qi, Jianxun
    Gao, George F.
    Shi, Yi
    NATURE, 2022, 610 (7931) : 394 - +
12345