Motion-resolved quantitative phase imaging

被引:10
|
作者
Kellman, Michael [1 ]
Chen, Michael [1 ]
Phillips, Zachary F. [2 ]
Lustig, Michael [1 ]
Waller, Laura [1 ]
机构
[1] Univ Calif Berkeley, Dept Elect Engn & Comp Sci, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Appl Sci & Technol, Berkeley, CA 94720 USA
来源
BIOMEDICAL OPTICS EXPRESS | 2018年 / 9卷 / 11期
基金
美国国家科学基金会;
关键词
SCANNING OPTICAL MICROSCOPY; REGISTRATION; CONTRAST; ILLUMINATION; RESOLUTION; BRAIN;
D O I
10.1364/BOE.9.005456
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The temporal resolution of quantitative phase imaging with Differential Phase Contrast (DPC) is limited by the requirement for multiple illumination-encoded measurements. This inhibits imaging of fast-moving samples. We present a computational approach to model and correct for non-rigid sample motion during the DPC acquisition in order to improve temporal resolution to that of a single-shot method and enable imaging of motion dynamics at the framerate of the sensor. Our method relies on the addition of a simultaneously-acquired color-multiplexed reference signal to enable non-rigid registration of measurements prior to phase retrieval. We show experimental results where we reduce motion blur from fast-moving live biological samples. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
引用
收藏
页码:5456 / 5466
页数:11
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