Antigen-capture ELISA for the detection of Rift Valley fever virus nucleoprotein using new monoclonal antibodies

被引：24
|
作者：
Fukushi, Shuetsu [1 ]
Nakauchi, Mina [1 ]
Mizutani, Tetsuya [1 ]
Saijo, Masayuki [1 ]
Kurane, Ichiro [1 ]
Morikawa, Shigeru [1 ]
机构：
[1] Natl Inst Infect Dis, Dept Virol 1, Tokyo 2080011, Japan
来源：
JOURNAL OF VIROLOGICAL METHODS | 2012年 / 180卷 / 1-2期
关键词：
Rift Valley fever virus; Monoclonal antibody; Antigen-capture ELISA; LINKED-IMMUNOSORBENT-ASSAY; RECENT COMMON ANCESTRY; RECOMBINANT NUCLEOPROTEIN; NUCLEOCAPSID PROTEIN; SAUDI-ARABIA; IGM ANTIBODIES; N-PROTEIN; EPIDEMIC; EXPRESSION; INFLUENZA;
D O I：
10.1016/j.jviromet.2011.12.013
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Monoclonal antibodies (MAbs) raised against the nucleoprotein (NP) of Rift Valley fever virus (RVFV) were developed, and an antigen-capture enzyme-linked immunosorbent assay (Ag-capture ELISA) system was developed for the detection of RVFV NP. The assay detected RVFV antigen from culture supernatants containing as little as 7.8-31.3 pfu per 100 mu l Reactivity with various truncated NPs indicated that MAb C10-54 bound only to the full-length NP, probably due to recognition of a conformational epitope, whereas MAbs G2-36 and D5-59 bound to a linear epitope ranging from amino acid residues 195-201 in the C-terminal region. Based on the alignments of the amino acid sequence of RVFV NP, the epitope regions of MAbs G2-36 and D5-59 were completely conserved among all RVFV strains. These results suggest that the MAbs are applicable to the Ag-capture ELISA for the diagnosis of RVFV infections. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：68 / 74
页数：7
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