Comparison of a Recombinant Nucleocapsid IgG Indirect ELISA with an IgG Sandwich ELISA for the Detection of Antibodies to Rift Valley Fever Virus in Small Ruminants

被引：7
|
作者：
Fafetine, Jose M. [1 ]
van Vuren, Petrus Jansen [2 ]
Paweska, Janusz T. [2 ]
机构：
[1] Eduardo Mondlane Univ, Fac Vet, Maputo, Mozambique
[2] Ctr Emerging & Zoonot Dis, Natl Inst Communicable Dis, Natl Hlth Lab Serv, Johannesburg, Gauteng, South Africa
来源：
VECTOR-BORNE AND ZOONOTIC DISEASES | 2012年 / 12卷 / 12期
关键词：
Antibodies; Enzyme-linked immunosorbent assay; Rift Valley fever virus; N-PROTEIN;
D O I：
10.1089/vbz.2012.1006
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Serological tests are widely used for Rift Valley fever (RVF) surveillance, disease control, and monitoring of immunological responses after vaccination. In recent years, several enzyme-linked immunosorbent assay (ELISA) formats have been developed for the detection of antibodies to RVF virus, but limited comparisons of their diagnostic sensitivities are available for specifically-targeted animal populations. This article describes the comparison of the commercially-available RVF recombinant nucleocapsid IgG indirect ELISA with the IgG sandwich ELISA in field-collected serum samples from 1262 domestic small ruminants in Mozambique. The agreement between the two tests measured by Cohen's kappa value was high (0.99 in goats and 0.92 in sheep), but the IgG sandwich ELISA was slightly more sensitive than the recombinant nucleocapsid IgG indirect ELISA in detecting the IgG response in sheep and goats naturally exposed to RVF virus.
引用
收藏
页码：1062 / 1064
页数：3
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