Identification of virulence genes in Campylobacter jejuni and C-coli isolates by PCR

被引:0
|
作者
Wieczorek, Kinga [1 ]
Osek, Jacek [1 ]
机构
[1] Natl Inst Vet Res, Dept Hyg Food Anim Origin, PL-24100 Pulawy, Poland
关键词
Campylobacter; virulence markers; detection; PCR;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Using the PCR technique, the presence of the seven genotypic virulence markers of Campylobacter has been tested. It was found that all C coli (92 strains) and C jejuni (105 strains) possessed the flaA and cadF markers. Several strains were positive for the CDT toxin genes (76.6%, 85.3%, and 83.2% strains with the A, B, and C cdt gene variants, respectively). On the other hand, only 66.0% and 20.8% isolates had the iam and virB genes.
引用
收藏
页码:211 / 216
页数:6
相关论文
共 50 条
  • [31] Comparison of Established PCR Assays for Accurate Identification of Campylobacter jejuni and Campylobacter coli
    Kabir, S. M. Lutful
    Chowdhury, Nityananda
    Asakura, Masahiro
    Shiramaru, Sachi
    Kikuchi, Ken
    Hinenoya, Atsushi
    Neogi, Sucharit Basu
    Yamasaki, Shinji
    [J]. JAPANESE JOURNAL OF INFECTIOUS DISEASES, 2019, 72 (02) : 81 - 87
  • [32] ENTEROTOXIGENICITY AND FREQUENCY OF CAMPYLOBACTER-JEJUNI, C-COLI AND C-LARIDIS IN HUMAN AND ANIMAL STOOL ISOLATES FROM DIFFERENT COUNTRIES
    LINDBLOM, GB
    JOHNY, M
    KHALIL, K
    MAZHAR, K
    RUIZPALACIOS, GM
    KAIJSER, B
    [J]. FEMS MICROBIOLOGY LETTERS, 1990, 66 (1-3) : 163 - 167
  • [33] Comparative performance of different PCR assays for the identification of Campylobacter jejuni and Campylobacter coli
    Debruyne, Lies
    Samyn, Ernly
    De Brandt, Evie
    Vandenberg, Olivier
    Heyndrickx, Marc
    Vandamme, Peter
    [J]. RESEARCH IN MICROBIOLOGY, 2008, 159 (02) : 88 - 93
  • [34] Conventional PCR- and real-time PCR-method for detection of thermophile Campylobacter jejuni, C-coli and C-lari:: An overview
    Lick, S.
    Mayr, A.
    Mueller, M.
    Anderson, A.
    Hotzel, H.
    Huber, I.
    [J]. JOURNAL FUR VERBRAUCHERSCHUTZ UND LEBENSMITTELSICHERHEIT-JOURNAL OF CONSUMER PROTECTION AND FOOD SAFETY, 2007, 2 (02): : 161 - 170
  • [35] Core Genome Multilocus Sequence Typing Scheme for Stable, Comparative Analyses of Campylobacter jejuni and C-coli Human Disease Isolates
    Cody, Alison J.
    Bray, James E.
    Jolley, Keith A.
    McCarthy, Noel D.
    Maiden, Martin C. J.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2017, 55 (07) : 2086 - 2097
  • [36] The changing patterns of β-lactamase activity amongst Campylobacter jejuni and C-coli in Lagos, Nigeria
    Smith, SI
    Coker, AO
    Olukoya, DK
    [J]. BIOMEDICAL LETTERS, 1997, 56 (223-24): : 179 - 181
  • [37] Inter-laboratory evaluation of three flagellin PCR/RFLP methods for typing Campylobacter jejuni and C-coli:: the CAMPYNET experience
    Harrington, CS
    Moran, L
    Ridley, AM
    Newell, DG
    Madden, RH
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2003, 95 (06) : 1321 - 1333
  • [38] Comparison of PCR methods for detection and speciation of Campylobacter jejuni and C-coli in chicken caecal contents against culture based methods
    Rodgers, J. D.
    Vidal, A.
    Ellis-Iversen, J.
    Ridley, A.
    [J]. ZOONOSES AND PUBLIC HEALTH, 2007, 54 : 42 - 42
  • [39] Detection of seven virulence and toxin genes of Campylobacter jejuni isolates from Danish turkeys by PCR and cytolethal distending toxin production of the isolates
    Bang, DD
    Borck, B
    Nielsen, EM
    Scheutz, F
    Pedersen, K
    Madsen, M
    [J]. JOURNAL OF FOOD PROTECTION, 2004, 67 (10) : 2171 - 2177
  • [40] Prevalence of Four Virulence Genes in Campylobacter jejuni Determined by PCR and Sequence Analysis
    Vasilios Kordinas
    Chryssoula Nicolaou
    Anastassios Ioannidis
    Eleni Papavasileiou
    Nicolaos John Legakis
    Stylianos Chatzipanagiotou
    [J]. Molecular Diagnosis, 2005, 9 (4) : 211 - 215