rpoB gene mutations in rifampin-resistant Mycobacterium tuberculosis identified by polymerase chain reaction single-stranded conformational polymorphism

被引：58
|
作者：
Bobadilla-del-Valle, M
Ponce-de-Leon, A
Axenas-Huertero, C
Vargas-Alarcon, G
Kato-Maeda, M
Small, PM
Couary, P
Ruiz-Palacios, GM
Sifuentes-Osornio, J
机构：
[1] Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Dept Infectol, Mexico City 14000, DF, Mexico
[2] Inst Nacl Cardiol Ignacio Chavez, Mexico City, DF, Mexico
[3] Univ Panamericana, Mexico City, DF, Mexico
[4] Stanford Univ, Med Ctr, Stanford, CA 94305 USA
来源：
EMERGING INFECTIOUS DISEASES | 2001年 / 7卷 / 06期
关键词：
D O I：
10.3201/eid0706.010615
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The use of polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) to study rpoB gene mutations in rifampin-resistant (RIFr) Mycobacterium tuberculosis has yielded contradictory results. To determine the sensitivity of this method, we analyzed 35 RIFr strains and 11 rifampin-susceptible (RIFs) strains, using the DNA sequencing of the core region of rpoB for comparison. Of the RIFr, 24 had a PCR-SSCP pattern identical to that of H37Rv; the other 11 had four different patterns. The 11 RIFs had PCR-SSCP patterns identical to that of H37Rv. The sensitivity of the assay was 31.4%; its specificity was 100%. We observed a strong correlation between the degree of resistance and the type of mutation.
引用
收藏
页码：1010 / 1013
页数：4
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