Mutations in the rpoB gene of Mycobacterium tuberculosis that interfere with PCR-single-strand conformation polymorphism analysis for rifampin susceptibility testing

被引:54
|
作者
Kim, BJ
Kim, SY
Park, BH
Lyu, MA
Park, IK
Bai, GH
Kim, SJ
Cha, CY
Kook, YH
机构
[1] SEOUL NATL UNIV,COLL MED,DEPT MICROBIOL,SEOUL 110799,SOUTH KOREA
[2] SEOUL NATL UNIV,COLL MED,CANC RES CTR,SEOUL 110799,SOUTH KOREA
[3] KOREAN NATL TB ASSOC,KOREAN INST TB,SEOUL,SOUTH KOREA
[4] CHUNGBUK NATL UNIV,COLL MED,DEPT MICROBIOL,CHEONGJU,SOUTH KOREA
关键词
D O I
10.1128/JCM.35.2.492-494.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Rifampin susceptibility of 32 rifampin-resistant and 26 rifampin-susceptible Mycobacterium tuberculosis strains was analyzed by PCR-single-strand conformation polymorphism (SSCP) and DNA sequencing within the 157-bp region of the rpoB gene (Ala(500) to Val(550)). Two false-positive PCR-SSCP results were observed among the susceptible strains due to the silent mutation Gln(513) (CAA-->CAG) and the deletion mutation Thr(508) and Ser(509). Another silent mutation [Leu(511) (CTG-->CTA)], combined with the mutation Ser(531)-->Leu, was observed in a resistant strain. These results suggest that to rule out false-positive PCR-SSCP results, sequencing of the target DNA is required.
引用
收藏
页码:492 / 494
页数:3
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