Molecular analysis of rifampin-resistant Mycobacterium tuberculosis isolated from Korea by polymerase chain reaction-single strand conformation polymorphism sequence analysis

被引：0
|
作者：
Lee, H
Cho, SN
Bang, HE
Lee, JH
Bae, GH
Kim, SJ
Kim, JD
机构：
[1] Yonsei Univ, Coll Med, Dept Microbiol, Inst Immunol & Immunol Dis, Seoul 120752, South Korea
[2] Korean Natl TB Assoc, Korean Inst TB, Seoul, South Korea
来源：
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | 1998年 / 2卷 / 07期
关键词：
M-tuberculosis; rifampin resistance; PCR-SSCP-sequence analysis; rpoB;
D O I：
暂无
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
OBJECTIVE: To assess the molecular mechanism of rifampin (RMP) resistance in clinical strains of Mycobacterium tuberculosis. DESIGN: The molecular nature of a part of the rpoB gene in 77 M. tuberculosis clinical strains isolated in Korea was analyzed using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and PCR-sequence analysis. RESULTS: Among 67 RMP-resistant isolates, 50 showed SSCP profiles different from that of an RMP-sensitive control strain, M. tuberculosis H37Rv, indicating the possible existence of a sequence alteration in this region of the rpoB gene, while 17 resistant isolates displayed SSCP profiles indistinguishable from that of the sensitive control strain. Subsequently, 17 clinical isolates whose SSCP profiles were difficult to distinguish from the control strain were subjected to sequence analysis. The analysis revealed that all 17 isolates did indeed contain mutations in the 81 bp region of the rpoB gene, which is associated with RMP resistance. CONCLUSION: The results from our study clearly indicate that the molecular mechanism of RMP resistance in M. tuberculosis isolates from Korea involves alterations in the rpoB gene. In addition, this study suggests that PCR-direct sequence analysis works more efficiently and accurately than PCR-SSCP analysis for rapid screening of RMP-resistant M, tuberculosis clinical isolates.
引用
收藏
页码：585 / 589
页数：5
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