Rotation of single bacterial cells relative to the optical axis using optical tweezers

被引：50

作者：

Carmon, G. ^{[1
,2
]}

Feingold, M. ^{[1
,2
]}

机构：

[1] Ben Gurion Univ Negev, Dept Phys, IL-84105 Beer Sheva, Israel

[2] Ben Gurion Univ Negev, Ilse Katz Ctr Nanotechnol, IL-84105 Beer Sheva, Israel

来源：

OPTICS LETTERS | 2011年 / 36卷 / 01期

关键词：

TRAPPED MICROSCOPIC PARTICLES; BIREFRINGENT MICRO-OBJECTS; ANGULAR-MOMENTUM TRANSFER; LASER-BEAMS; ALIGNMENT; MANIPULATION; LIGHT; MOLECULES; DYNAMICS;

D O I：

10.1364/OL.36.000040

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Using a single-beam, oscillating optical tweezers, we demonstrate trapping and rotation of rod-shaped bacterial cells with respect to the optical axis. The angle of rotation, theta, is determined by the amplitude of the oscillation. It is shown that theta can be measured from the longitudinal cell intensity profiles in the corresponding phase-contrast images. The technique allows viewing the cell from different perspectives and can provide a useful tool in fluorescence microscopy for the analysis of three-dimensional subcellular structures. (C) 2010 Optical Society of America

引用

页码：40 / 42

页数：3

共 50 条

[41] Single cell manipulation by using tilt controlled optical tweezers
Ichikawa, Masatoshi
Kubo, Koji
Murata, Shizuaki
Yoshikawa, Kenichi
Kimura, Yasuyuki
2007 INTERNATIONAL SYMPOSIUM ON MICRO-NANO MECHATRONICS AND HUMAN SCIENCE, VOLS 1 AND 2, 2007, : 316 - +
[42] Single molecule myosin mechanics examined using optical tweezers
Mehta, AD
Spudich, JA
BIOPHYSICAL JOURNAL, 1998, 74 (02) : A225 - A225
[43] Enantioselective manipulation of single chiral nanoparticles using optical tweezers
Ali, Rfaqat
Pinheiro, Felipe A.
Dutra, Rafael S.
Rosa, Felipe S.
Maia Neto, Paulo A.
NANOSCALE, 2020, 12 (08) : 5031 - 5037
[44] Rotation of birefringent particles in optical tweezers with spherical aberration
Zhong, Min-Cheng
Zhou, Jin-Hua
Ren, Yu-Xuan
Li, Yin-Mei
Wang, Zi-Qiang
APPLIED OPTICS, 2009, 48 (22) : 4397 - 4402
[45] Rapid rotation of micron and submicron dielectric particles measured using optical tweezers
Rowe, AD
Leake, MC
Morgan, H
Berry, RM
JOURNAL OF MODERN OPTICS, 2003, 50 (10) : 1539 - 1554
[46] Controlled rotation and orientation of rubrene particles and Escherichia coli using optical tweezers
Li, X. C.
Sun, X. D.
LASER PHYSICS, 2010, 20 (08) : 1774 - 1777
[47] Rotation dynamics of yeast cell in vortex optical tweezers
Gao H.
Ren Y.
Liu W.
Li Y.
Zhongguo Jiguang/Chinese Journal of Lasers, 2011, 38 (04):
[48] Use of shape induced birefringence for rotation in optical tweezers
Asavei, Theodor
Nieminen, Timo A.
Heckenberg, Norman R.
Rubinsztein-Dunlop, Halina
OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION VII, 2010, 7762
[49] Optically controlled rotation of PTCDA crystals in optical tweezers
Starr, C
Dultz, W
Wagner, HP
Dholakia, K
Schmitzer, H
Physics of Semiconductors, Pts A and B, 2005, 772 : 1099 - 1100
[50] Optical confinement efficiency in the single beam intracavity optical tweezers
Kuang, Tengfang
Xiong, Wei
Luo, Bin
Chen, Xinlin
Liu, Zijie
Han, Xiang
Xiao, Guangzong
Yang, Kaiyong
Luo, Hui
OPTICS EXPRESS, 2020, 28 (24): : 35734 - 35747

← 1 2 3 4 5 →