Rotation of single bacterial cells relative to the optical axis using optical tweezers

被引：50
|
作者：
Carmon, G. [1 ,2 ]
Feingold, M. [1 ,2 ]
机构：
[1] Ben Gurion Univ Negev, Dept Phys, IL-84105 Beer Sheva, Israel
[2] Ben Gurion Univ Negev, Ilse Katz Ctr Nanotechnol, IL-84105 Beer Sheva, Israel
来源：
OPTICS LETTERS | 2011年 / 36卷 / 01期
关键词：
TRAPPED MICROSCOPIC PARTICLES; BIREFRINGENT MICRO-OBJECTS; ANGULAR-MOMENTUM TRANSFER; LASER-BEAMS; ALIGNMENT; MANIPULATION; LIGHT; MOLECULES; DYNAMICS;
D O I：
10.1364/OL.36.000040
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Using a single-beam, oscillating optical tweezers, we demonstrate trapping and rotation of rod-shaped bacterial cells with respect to the optical axis. The angle of rotation, theta, is determined by the amplitude of the oscillation. It is shown that theta can be measured from the longitudinal cell intensity profiles in the corresponding phase-contrast images. The technique allows viewing the cell from different perspectives and can provide a useful tool in fluorescence microscopy for the analysis of three-dimensional subcellular structures. (C) 2010 Optical Society of America
引用
收藏
页码：40 / 42
页数：3
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