A new interference-free lysine biosensor using a non-conducting polymer film

被引：43
|
作者：
Curulli, A
Kelly, S
O'Sullivan, C
Guilbault, GG
Palleschi, G
机构：
[1] Studio Elettrochim & Chim Fis Interfasi, Ctr CNR, I-00161 Rome, Italy
[2] Natl Univ Ireland Univ Coll Cork, Cork, Ireland
[3] Univ Roma Tor Vergata, Dipartimento Sci & Tecnol Chim, Rome, Italy
来源：
BIOSENSORS & BIOELECTRONICS | 1998年 / 13卷 / 12期
关键词：
non-conducting polymers; electropolymerisation; interferences; lysine; food analysis; lysine oxidase;
D O I：
10.1016/S0956-5663(98)00075-X
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
An electrochemical biosensor for the determination of lysine to be used for rapid evaluation of food quality has been developed. Platinum electrodes have been coated by electropolymerisation with 1,2-diaminobenzene( 1.2-DAB) using cyclic voltammetry. The reduction in the oxidation of interferents compared with the bare platinum electrode was 100% for ascorbic acid, 99% for acetaminophen and 99% for cysteine. The enzyme L-lysine-cu-oxidase was then immobilised onto the polymer layer by passive adsorption and a calibration curve for lysine constructed. This gave a linear range of 1 x 10(-5) mol/l to 1 x 10(-3) mol/l and a limit of detection of 2 x 10(-7) mel/l. (C) 1998 Elsevier Science S.A. All rights reserved.
引用
收藏
页码：1245 / 1250
页数：6
相关论文
共 50 条
  • [1] A new interference-free lysine biosensor using a non-conducting polymer film
    Curulli, A.
    Kelly, S.
    O'Sullivan, C.
    Guilbault, G.G.
    Palleschi, G.
    Biosensors and Bioelectronics, 1998, 13 (12): : 1245 - 1250
  • [2] A new interference-free lysine biosensor using a non-conducting polymer film (vol 13, pg 1245, 1998)
    Kelly, S
    Curulli, A
    O'Sullivan, C
    Guilbault, GG
    Palleschi, G
    BIOSENSORS & BIOELECTRONICS, 1999, 14 (03): : 353 - 353
  • [3] An interference-free first generation alcohol biosensor based on a gold electrode modified by an overoxidised non-conducting polypyrrole film
    Carelli, D
    Centonze, D
    De Giglio, A
    Quinto, M
    Zambonin, PG
    ANALYTICA CHIMICA ACTA, 2006, 565 (01) : 27 - 35
  • [4] Disposable interference-free glucose biosensor based on an electropolymerised poly(pyrrole) permselective film
    Quinto, M
    Losito, I
    Palmisano, F
    Zambonin, CG
    ANALYTICA CHIMICA ACTA, 2000, 420 (01) : 9 - 17
  • [5] An interference-free glucose biosensor based on a novel low potential redox polymer mediator
    Deng, Huimin
    Teo, Alan Kay Liang
    Gao, Zhiqiang
    SENSORS AND ACTUATORS B-CHEMICAL, 2014, 191 : 522 - 528
  • [6] Electrochemical immobilisation of enzymes on conducting organic salt electrodes: characterisation of an oxygen independent and interference-free glucose biosensor
    Centonze, D
    Losito, I
    Malitesta, C
    Palmisano, F
    Zambonin, PG
    JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1997, 435 (1-2): : 103 - 111
  • [7] Electrochemical immobilization of enzymes on conducting organic salt electrodes: Characterization of an oxygen independent and interference-free glucose biosensor
    Universita degli Studi della, Basilicata, Potenza, Italy
    J Electroanal Chem, 1-2 (103-111):
  • [8] ELECTROCHEMICAL IMMOBILIZATION OF ENZYMES ON CONDUCTING ORGANIC SALT ELECTRODES - PREPARATION OF AN OXYGEN INDEPENDENT AND INTERFERENCE-FREE GLUCOSE BIOSENSOR
    PALMISANO, F
    CENTONZE, D
    MALITESTA, C
    ZAMBONIN, PG
    JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1995, 381 (1-2): : 235 - 237
  • [9] An Interference-Free Glucose Biosensor Based on an Anionic Redox Polymer-Mediated Enzymatic Oxidation of Glucose
    Deng, Huimin
    Shen, Wei
    Gao, Zhiqiang
    CHEMPHYSCHEM, 2013, 14 (10) : 2343 - 2347
  • [10] Protein and polysaccharide-composite sol-gel silicate film for an interference-free amperometric glucose biosensor
    Matsuhisa, Hironori
    Tsuchiya, Munenori
    Hasebe, Yasushi
    COLLOIDS AND SURFACES B-BIOINTERFACES, 2013, 111 : 523 - 529
12345