Prevention of cross-contamination during sampling procedure in molecular detection for cancer micrometastasis

被引：7
|
作者：
Fujiwara, Y [1 ]
Ooka, M [1 ]
Sugita, Y [1 ]
Sakita, I [1 ]
Tamaki, Y [1 ]
Monden, M [1 ]
机构：
[1] Osaka Univ, Grad Sch Med, Dept Surg & Clin Oncol, Suita, Osaka 5650871, Japan
来源：
CANCER LETTERS | 2000年 / 153卷 / 1-2期
关键词：
micrometastasis; reverse transcriptase-polymerase chain reaction; cross-contamination; lymph node; breast cancer;
D O I：
10.1016/S0304-3835(00)00355-4
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Reverse transcriptase-polymerase chain reaction (RT-PCR) techniques have been widely employed as an ultra-sensitive method for detection of micrometastases in patients with various types of malignancies. Messenger RNA of a specific marker gene is a target for RT-PCR amplification to examine the presence of micrometastases in body fluids or tissues obtained from human. We developed the RT-PCR assay specific for rat p-actin mRNA, which cannot detect human counterpart and assessed how much contamination of rat tissues can influence the result of RT-PCR assay and how to avoid the influence of the contamination in RT-PCR assay. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：109 / 111
页数：3
相关论文
共 50 条
  • [41] MonoPrep® processing of cytology specimens prevents cellular and molecular cross-contamination
    Bolick, David
    Owens, Marty
    Costello, Maureen
    Friedman, Marc
    Gamerman, Gary
    CANCER CYTOPATHOLOGY, 2006, 108 (05): : 398 - 398
  • [42] An artificial intelligent platform for live cell identification and the detection of cross-contamination
    Wang, Ruixin
    Wang, Dongni
    Kang, Dekai
    Guo, Xusen
    Guo, Chong
    Dongye, Meimei
    Zhu, Yi
    Chen, Chuan
    Zhang, Xiayin
    Long, Erping
    Wu, Xiaohang
    Liu, Zhenzhen
    Lin, Duoru
    Wang, Jinghui
    Huang, Kai
    Lin, Haotian
    ANNALS OF TRANSLATIONAL MEDICINE, 2020, 8 (11)
  • [43] Sensitivity of isoenzyme analysis for the detection of interspecies cell line cross-contamination
    Nims, RW
    Shoemaker, AP
    Bauernschub, MA
    Rec, LJ
    Harbell, JW
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 1998, 34 (01) : 35 - 39
  • [44] Dissipation and cross-contamination of miticides in apiculture. Evaluation by APIStrip-based sampling
    Murcia-Morales, Maria
    Jose Diaz-Galiano, Francisco
    Guiterrez-Tirado, Inmaculada
    Manuel Flores, Jose
    Van der Steen, Jozef J. M.
    Fernandez-Alba, Amadeo R.
    CHEMOSPHERE, 2021, 280
  • [45] Evidence for false-positive results for Boldenone testing of veal urine due to faecal cross-contamination during sampling
    Rossi, CAS
    Arioli, F
    Bassini, A
    Chiesa, LM
    Dell'Orto, V
    Montana, M
    Pompa, G
    FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2004, 21 (08): : 756 - 762
  • [46] Modeling cross-contamination during poultry processing: Dynamics in the chiller tank
    Munther, Daniel
    Sun, Xiaodan
    Xiao, Yanni
    Tang, Sanyi
    Shimozako, Helio
    Wu, Jianhong
    Smith, Ben A.
    Fazil, Aamir
    FOOD CONTROL, 2016, 59 : 271 - 281
  • [47] Investigation into cross-contamination during cleaning efficiency testing in PET recycling
    Welle, Frank
    RESOURCES CONSERVATION AND RECYCLING, 2016, 112 : 65 - 72
  • [48] Microbial cross-contamination by airborne dispersion and contagion during defeathering of poultry
    Allen, VM
    Hinton, MH
    Tinker, DB
    Gibson, C
    Mead, GC
    Wathes, CM
    BRITISH POULTRY SCIENCE, 2003, 44 (04) : 567 - 576
  • [49] Simulation Model for Campylobacter Cross-Contamination During Poultry Processing at Slaughterhouses
    Hayama, Y.
    Yamamoto, T.
    Kasuga, F.
    Tsutsui, T.
    ZOONOSES AND PUBLIC HEALTH, 2011, 58 (06) : 399 - 406
  • [50] Bacterial cross-contamination of meat during liquid nitrogen immersion freezing
    Berry, ED
    Dorsa, WJ
    Siragusa, GR
    Koohmaraie, M
    JOURNAL OF FOOD PROTECTION, 1998, 61 (09) : 1103 - 1108
12345