Prevention of cross-contamination during sampling procedure in molecular detection for cancer micrometastasis

被引：7
|
作者：
Fujiwara, Y [1 ]
Ooka, M [1 ]
Sugita, Y [1 ]
Sakita, I [1 ]
Tamaki, Y [1 ]
Monden, M [1 ]
机构：
[1] Osaka Univ, Grad Sch Med, Dept Surg & Clin Oncol, Suita, Osaka 5650871, Japan
来源：
CANCER LETTERS | 2000年 / 153卷 / 1-2期
关键词：
micrometastasis; reverse transcriptase-polymerase chain reaction; cross-contamination; lymph node; breast cancer;
D O I：
10.1016/S0304-3835(00)00355-4
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Reverse transcriptase-polymerase chain reaction (RT-PCR) techniques have been widely employed as an ultra-sensitive method for detection of micrometastases in patients with various types of malignancies. Messenger RNA of a specific marker gene is a target for RT-PCR amplification to examine the presence of micrometastases in body fluids or tissues obtained from human. We developed the RT-PCR assay specific for rat p-actin mRNA, which cannot detect human counterpart and assessed how much contamination of rat tissues can influence the result of RT-PCR assay and how to avoid the influence of the contamination in RT-PCR assay. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：109 / 111
页数：3
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