Imaging of Chromosomes at Nanometer-Scale Resolution Using Soft X-Ray Microscope at Ritsumeikan University SR Center

被引：0

作者：

Yamamoto, A. ^{[1
]}

Takemoto, K. ^{[2
]}

Komura, I. ^{[3
]}

Namba, H. ^{[4
]}

Kihara, H. ^{[4
]}

机构：

[1] Nagahama Inst Biosci & Technol, 1266 Tamura Cho, Shiga 5260829, Japan

[2] Kansai Med Univ, Dept Phys, Osaka 5731136, Japan

[3] Ritsumeikan Univ, Dept Phys Sci, Shiga 5258577, Japan

[4] Ritsumeikan Univ, SR Ctr, Shiga 5258577, Japan

来源：

9TH INTERNATIONAL CONFERENCE ON X-RAY MICROSCOPY | 2009年 / 186卷

关键词：

D O I：

10.1088/1742-6596/186/1/012098

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

In order to clarify the process of condensation and distribution of a chromosome with 70 nm resolution, process of cell division was observed by soft X-ray microscopy. During prometaphase of mouse fibroblast cell line NIH3T3, each chromosome is clearly visualized at high contrast. Furthermore, thickness of a chromosome is not uniform but varied from 150 nm to 750 nm. This is considered as a condensing step of chromatin fiber occurring in the prometaphase. During metaphase and anaphase, each chromosome was also observed. The chromosomes were thicker than those in prometaphase. This result implies that during metaphase and anaphase the chromosomes condense and become more compact.

引用

页数：3

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