The study of a monocotyledon abscission zone using microscopic, chemical, enzymatic and solid state 13C CP/MAS NMR analyses

被引:30
|
作者
Henderson, J
Davies, HA
Heyes, SJ
Osborne, DJ
机构
[1] Open Univ, Oxford Res Unit, Oxford OX1 5HR, England
[2] Open Univ, Dept Biol Sci, Milton Keynes MK7 6AA, Bucks, England
[3] Univ Oxford, Dept Chem, Inorgan Chem Lab, Oxford OX1 3QR, England
关键词
Elaeis guincensis Jacq; oil palm; abscission; pectin; uronic acids lignin; polygalacturonase; cellulase;
D O I
10.1016/S0031-9422(00)00447-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have investigated distinguishing features in cells of the abscission zone of a monocotyledon fruit, the oil palm Elaeis guineensis. The cell walls of the abscission zone and the subtending mesocarp and pedivel have been analysed by light and transmission electron microscopy, by chemical methods and by solid state C-13 CP/MAS NMR spectroscopy. Results show that these abscission zone cells have specific characteristics which include high levels of unmethylated pectin in the walls and an inducible (x35) polygalacturonase enzyme expression. Together these findings help to explain the localised precision of cell separation events. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:131 / 139
页数:9
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