Reprogramming of one human induced pluripotent stem cell line from healthy donor

被引：1
|
作者：
Cao, Shuxing [1 ]
Ma, Jun [2 ,3 ,4 ]
Zhang, Jinyu [2 ,3 ]
Guo, Ruiyun [2 ,3 ]
Liu, Xin [2 ,3 ]
Li, Ming [1 ,2 ,3 ,5 ]
机构：
[1] Hebei Med Univ, Hosp 2, Hebei 050017, Peoples R China
[2] Hebei Med Univ, Natl Univ Ireland Galway Stem Cell Res Ctr, Hebei 050017, Peoples R China
[3] Hebei Res Ctr Stem Cell Med Translat Engn, Hebei 050017, Peoples R China
[4] Hebei Med Univ, Human Anat Dept, Hebei 050017, Peoples R China
[5] Hebei Med Univ, Hosp 2, Natl Univ Ireland Galway Stem Cell Res Ctr, Hebei Res Ctr Stem Cell Med Translat Engn, Hebei 050017, Peoples R China
来源：
STEM CELL RESEARCH | 2021年 / 57卷
关键词：
D O I：
10.1016/j.scr.2021.102613
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
In this study, skin biopsy was, and the fibroblasts were isolated from the dermal explant cultures. Human induced pluripotent stem cell (iPSC) line was generated from the skin fibroblasts collected from a healthy 50-year old male donor with informed consent. The reprogramming of fibroblasts was performed with four Yamanaka factors containing Oct3/4, Sox2, Klf4 and c-Myc. The generated iPSCs were confirmed integration-free, expressed pluripotency markers, displayed the normal karyotype, and demonstrated trilineage differentiation potential. This iPSC model can be used to model physiological processes and screen drug validation in vitro.
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页数：4
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