Reprogramming of one human induced pluripotent stem cell line from healthy donor

被引：1
|
作者：
Cao, Shuxing [1 ]
Ma, Jun [2 ,3 ,4 ]
Zhang, Jinyu [2 ,3 ]
Guo, Ruiyun [2 ,3 ]
Liu, Xin [2 ,3 ]
Li, Ming [1 ,2 ,3 ,5 ]
机构：
[1] Hebei Med Univ, Hosp 2, Hebei 050017, Peoples R China
[2] Hebei Med Univ, Natl Univ Ireland Galway Stem Cell Res Ctr, Hebei 050017, Peoples R China
[3] Hebei Res Ctr Stem Cell Med Translat Engn, Hebei 050017, Peoples R China
[4] Hebei Med Univ, Human Anat Dept, Hebei 050017, Peoples R China
[5] Hebei Med Univ, Hosp 2, Natl Univ Ireland Galway Stem Cell Res Ctr, Hebei Res Ctr Stem Cell Med Translat Engn, Hebei 050017, Peoples R China
来源：
STEM CELL RESEARCH | 2021年 / 57卷
关键词：
D O I：
10.1016/j.scr.2021.102613
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
In this study, skin biopsy was, and the fibroblasts were isolated from the dermal explant cultures. Human induced pluripotent stem cell (iPSC) line was generated from the skin fibroblasts collected from a healthy 50-year old male donor with informed consent. The reprogramming of fibroblasts was performed with four Yamanaka factors containing Oct3/4, Sox2, Klf4 and c-Myc. The generated iPSCs were confirmed integration-free, expressed pluripotency markers, displayed the normal karyotype, and demonstrated trilineage differentiation potential. This iPSC model can be used to model physiological processes and screen drug validation in vitro.
引用
收藏
页数：4
相关论文
共 50 条
  • [31] Generation of a human induced pluripotent stem cell line (SIAISi011-A) from a 61-year-old Chinese Han healthy female donor
    Zhang, Wenxin
    Hua, Yishi
    Di, Weihao
    Guo, Jinghui
    Dai, Qiuting
    Zhao, Jian
    Zhang, Bei
    Wang, Ying
    STEM CELL RESEARCH, 2021, 51
  • [32] RETRACTED: Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using integration free Sendai technology (Retracted Article)
    Rossbach, Bella
    Hildebrand, Laura
    El-Ahmad, Linda
    Stachelscheid, Harald
    Reinke, Petra
    Kurtz, Andreas
    STEM CELL RESEARCH, 2016, 16 (01) : 133 - 136
  • [33] Generation of the induced pluripotent stem cell line UHOMi002-A from peripheral blood mononuclear cells of a healthy male donor
    Fieldes, Mathieu
    Ahmed, Engi
    Bourguignon, Chloe
    Mianne, Joffrey
    Martin, Melanie
    Arnould, Cecile
    Vachier, Isabelle
    Assou, Said
    De Vos, John
    Bourdin, Arnaud
    STEM CELL RESEARCH, 2020, 49
  • [34] Induced pluripotent stem cell reprogramming of lymphoblastoid cell lines from Estonian individuals
    Kreevan, Rita
    Trei, Annika
    Liivand, Laura
    Eriksson, Jon Anders
    Romero, Irene Gallego
    Org, Tonis
    EUROPEAN JOURNAL OF HUMAN GENETICS, 2023, 31 : 301 - 302
  • [35] Generation of human induced pluripotent stem cell line from a patient with restrictive cardiomyopathy
    Li, Jingxian
    Jiang, Jinxiu
    Ye, Lingqun
    Lian, Zhipeng
    Gong, Hui
    Lei, Wei
    Dai, Yuxiang
    Hu, Shijun
    STEM CELL RESEARCH, 2024, 76
  • [36] Establishment of a human induced pluripotent stem cell line from a patient with dilated cardiomyopathy
    Ye, Lingqun
    Ni, Baoqiang
    Wu, Hongchun
    Han, Xinglong
    Li, Huadong
    Liu, Junwei
    Hu, Shijun
    Lei, Wei
    STEM CELL RESEARCH, 2024, 78
  • [37] Generation of a human induced pluripotent stem cell line from a patient with dent disease
    Fang, Xianying
    Kim, Ji Hyun
    Cui, Sheng
    Shin, Yoo Jin
    Lee, Hanbi
    Ko, Eun Jeong
    Cheong, Hae Il
    Kim, Sejoong
    Kim, Hoon Seok
    Kim, Myungshin
    Yang, Chul Woo
    Lim, Sun Woo
    Ha Chung, Byung
    STEM CELL RESEARCH, 2023, 71
  • [38] Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background
    Dai, Qiuting
    Di, Weihao
    Zhang, Yi
    Gu, Shixin
    Zhao, Jian
    Zhang, Bei
    Wang, Ying
    STEM CELL RESEARCH, 2021, 53
  • [39] An achievement has been made in establishing an induced pluripotent stem cell line (SDPHi005-A) from a healthy Chinese male donor
    Shao, Jing
    Jin, Yan
    Shao, Chunhong
    Zhang, Xiaoqiao
    Yang, Guang
    STEM CELL RESEARCH, 2024, 77
  • [40] Understanding epigenomic reprogramming in human induced pluripotent stem cells
    Laffman-Johnson, Elise
    CLINICAL PHARMACOLOGY & THERAPEUTICS, 2011, 89 (05) : 634 - 635
12345