Reprogramming of one human induced pluripotent stem cell line from healthy donor

被引：1

作者：

Cao, Shuxing ^{[1
]}

Ma, Jun ^{[2
,3
,4
]}

Zhang, Jinyu ^{[2
,3
]}

Guo, Ruiyun ^{[2
,3
]}

Liu, Xin ^{[2
,3
]}

Li, Ming ^{[1
,2
,3
,5
]}

机构：

[1] Hebei Med Univ, Hosp 2, Hebei 050017, Peoples R China

[2] Hebei Med Univ, Natl Univ Ireland Galway Stem Cell Res Ctr, Hebei 050017, Peoples R China

[3] Hebei Res Ctr Stem Cell Med Translat Engn, Hebei 050017, Peoples R China

[4] Hebei Med Univ, Human Anat Dept, Hebei 050017, Peoples R China

[5] Hebei Med Univ, Hosp 2, Natl Univ Ireland Galway Stem Cell Res Ctr, Hebei Res Ctr Stem Cell Med Translat Engn, Hebei 050017, Peoples R China

来源：

STEM CELL RESEARCH | 2021年 / 57卷

关键词：

D O I：

10.1016/j.scr.2021.102613

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

In this study, skin biopsy was, and the fibroblasts were isolated from the dermal explant cultures. Human induced pluripotent stem cell (iPSC) line was generated from the skin fibroblasts collected from a healthy 50-year old male donor with informed consent. The reprogramming of fibroblasts was performed with four Yamanaka factors containing Oct3/4, Sox2, Klf4 and c-Myc. The generated iPSCs were confirmed integration-free, expressed pluripotency markers, displayed the normal karyotype, and demonstrated trilineage differentiation potential. This iPSC model can be used to model physiological processes and screen drug validation in vitro.

引用

页数：4

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