Droplet DNA binding detection on microfluidic flow-focusing

被引：0
|
作者：
Kim, Sunggu [1 ]
Lee, Junghoon [1 ]
机构：
[1] Seoul Natl Univ, Dept Mech Engn, Seoul, South Korea
来源：
2015 IEEE SENSORS | 2015年
关键词：
flow-focusing; droplet DNA sensor; droplet interfacial control;
D O I：
暂无
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
We report the interfacial DNA binding by observing size shift of droplets in a flow-focusing device. Molecular binding at the phospholipid on oil-water interface causes interfacial energy change which alters the droplet production rate in the microchannel. Smaller droplets were produced at the orifice due to a complementary DNA binding compare with non-complementary DNA and control cases. Our report contains flow condition tuning for clear distinction of binding events and molecular protocol to modify the interface with single-strand (ss) DNA.
引用
收藏
页码：950 / 952
页数：3
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