PCR screening and sequence analysis of iol clusters in Lactobacillus casei strains isolated from koumiss

被引:6
|
作者
Zhang, W. [1 ]
Sun, Z. [1 ]
Sun, T. [1 ]
Zhang, H. [1 ]
机构
[1] Inner Mongolia Agr Univ, Sch Food Sci & Engn, Minist Educ, Key Lab Dairy Biotechnol & Engn, Hohhot 010018, Peoples R China
基金
中国国家自然科学基金;
关键词
MYOINOSITOL CATABOLISM; IDENTIFICATION; INOSITOL; GENE; DIVERSITY; PRODUCTS; BACTERIA; MILK;
D O I
10.1007/s12223-010-0097-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The iol cluster (consisting of genes involved in myo-inositol utilization) was investigated in Lactobacillus casei strains isolated from koumiss. Ten strains were tested for the presence of iol cluster by PCR screening; three strains encoded this cluster. Full-sequencing procedure was conducted; the iol cluster was identical to that of L. casei BL23 (GenBank access. no. FM177140) except for an upstream transposase. The iol cluster is not a common feature for L. casei strains isolated from koumiss.
引用
收藏
页码:603 / 606
页数:4
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