共 50 条
Differential expression of gut miRNAs in idiopathic Parkinson's disease
被引:8
|作者:
Kurz, Anna
[2
,10
]
Kumar, Rohit
[7
,10
]
Northoff, Bernd H.
[3
]
Wenk, Catharina
[3
]
Schirra, Joerg
[4
]
Donakonda, Sainitin
[8
]
Hoeglinger, Guenter U.
[5
,6
,10
]
Schwarz, Johannes
[9
]
Rozanski, Verena
[9
]
Huebner, Rainer
[9
]
Botzel, Kai
[2
]
Holdt, Lesca Miriam
[3
]
Koeglsperger, Thomas
[1
,2
]
机构:
[1] German Ctr Neurodegenerat Dis DZNE, Dept Translat Brain Res, Munich, Germany
[2] Ludwig Maximilians Univ Munchen, Dept Neurol, Munich, Germany
[3] Ludwig Maximilian Univ LMU Munich, Univ Hosp, Inst Lab Med, Munich, Germany
[4] Ludwig Maximilians Univ Munchen, Dept Internal Med 3, Munich, Germany
[5] Tech Univ, Dept Neurol, Munich, Germany
[6] Hannover Med Sch, Dept Neurol, Hannover, Germany
[7] Tech Univ Munich, Med Sch, Munich, Germany
[8] Tech Univ Munich, Inst Immunol & Expt Oncol, Munich, Germany
[9] Klin Haag I OB, Dept Neurol, Muhldorf, Germany
[10] German Ctr Neurodegenerat Dis DZNE, Dept Translat Neurodegenerat, Munich, Germany
关键词:
Parkinson's disease;
Lewy body disease;
Gut biopsy;
Biomarker;
microRNA;
ALPHA-SYNUCLEIN;
SYSTEM;
GENES;
D O I:
10.1016/j.parkreldis.2021.05.022
中图分类号:
R74 [神经病学与精神病学];
学科分类号:
摘要:
Objective: In the present work, we aimed to investigate the expression of microRNAs (miRNAs) in routine colonic biopsies obtained from patients with idiopathic Parkinson's disease (PD) and to address their value as a diagnostic biomarker for PD and their mechanistic contribution to PD onset and progression. Methods: Patients with PD (n = 13) and healthy controls (n = 17) were prospectively recruited to undergo routine colonic biopsies for cancer screening. Total RNA was extracted from the biopsy material and the expression of miRNAs was quantified by Illumina High-Throughput Sequencing. Results: Statistical analysis revealed a significant submucosal enrichment of the miRNA hsa-miR-486-5p in colonic biopsies from PD patients compared to the control subjects. The expression of miR-486-5p correlated with age and disease severity as measured by the UPDRS and Hoehn & Yahr scale. miRNA gene target analysis identified 301 gene targets that are affected by miR-486-5p. A follow-up associated target identification and pathway enrichment analysis further determined their role in distinct biological processes in the enteric nervous system (ENS). Interpretation: Our work demonstrates an enrichment of submucosal miR-486-5p in routine colonic biopsies from PD patients. Our results will support the examination of miR-486-5p as a PD biomarker and help to understand the significance of the miR-486-5p gene targets for PD onset and progression. In addition, our data will support the investigation of the molecular and cellular mechanisms of GI dysfunction in PD.
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页码:46 / 50
页数:5
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