Differential expression of gut miRNAs in idiopathic Parkinson's disease

被引:8
|
作者
Kurz, Anna [2 ,10 ]
Kumar, Rohit [7 ,10 ]
Northoff, Bernd H. [3 ]
Wenk, Catharina [3 ]
Schirra, Joerg [4 ]
Donakonda, Sainitin [8 ]
Hoeglinger, Guenter U. [5 ,6 ,10 ]
Schwarz, Johannes [9 ]
Rozanski, Verena [9 ]
Huebner, Rainer [9 ]
Botzel, Kai [2 ]
Holdt, Lesca Miriam [3 ]
Koeglsperger, Thomas [1 ,2 ]
机构
[1] German Ctr Neurodegenerat Dis DZNE, Dept Translat Brain Res, Munich, Germany
[2] Ludwig Maximilians Univ Munchen, Dept Neurol, Munich, Germany
[3] Ludwig Maximilian Univ LMU Munich, Univ Hosp, Inst Lab Med, Munich, Germany
[4] Ludwig Maximilians Univ Munchen, Dept Internal Med 3, Munich, Germany
[5] Tech Univ, Dept Neurol, Munich, Germany
[6] Hannover Med Sch, Dept Neurol, Hannover, Germany
[7] Tech Univ Munich, Med Sch, Munich, Germany
[8] Tech Univ Munich, Inst Immunol & Expt Oncol, Munich, Germany
[9] Klin Haag I OB, Dept Neurol, Muhldorf, Germany
[10] German Ctr Neurodegenerat Dis DZNE, Dept Translat Neurodegenerat, Munich, Germany
关键词
Parkinson's disease; Lewy body disease; Gut biopsy; Biomarker; microRNA; ALPHA-SYNUCLEIN; SYSTEM; GENES;
D O I
10.1016/j.parkreldis.2021.05.022
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Objective: In the present work, we aimed to investigate the expression of microRNAs (miRNAs) in routine colonic biopsies obtained from patients with idiopathic Parkinson's disease (PD) and to address their value as a diagnostic biomarker for PD and their mechanistic contribution to PD onset and progression. Methods: Patients with PD (n = 13) and healthy controls (n = 17) were prospectively recruited to undergo routine colonic biopsies for cancer screening. Total RNA was extracted from the biopsy material and the expression of miRNAs was quantified by Illumina High-Throughput Sequencing. Results: Statistical analysis revealed a significant submucosal enrichment of the miRNA hsa-miR-486-5p in colonic biopsies from PD patients compared to the control subjects. The expression of miR-486-5p correlated with age and disease severity as measured by the UPDRS and Hoehn & Yahr scale. miRNA gene target analysis identified 301 gene targets that are affected by miR-486-5p. A follow-up associated target identification and pathway enrichment analysis further determined their role in distinct biological processes in the enteric nervous system (ENS). Interpretation: Our work demonstrates an enrichment of submucosal miR-486-5p in routine colonic biopsies from PD patients. Our results will support the examination of miR-486-5p as a PD biomarker and help to understand the significance of the miR-486-5p gene targets for PD onset and progression. In addition, our data will support the investigation of the molecular and cellular mechanisms of GI dysfunction in PD.
引用
收藏
页码:46 / 50
页数:5
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