CHARACTERIZATION OF LEPTOSPIRA BORGPETERSENII ISOLATES FROM FIELD RATS (RATTUS NORVEGICUS) BY 16S RRNA AND LIPL32 GENE SEQUENCING

被引：0
|
作者：
Vedhagiri, Kumaresan [1 ]
Natarajaseenivasan, Kalimuthusamy [1 ]
Prabhakaran, Shanmugarajan G. [1 ]
Selvin, Joseph [1 ]
Narayanan, Ramasamy [1 ]
Shouche, Yogesh S. [2 ]
Vijayachari, Paluru [3 ]
Ratnam, Sivalingam [4 ]
机构：
[1] Bharathidasan Univ, Sch Life Sci, Dept Microbiol, Div Med Microbiol, Tiruchirappalli 620024, Tamil Nadu, India
[2] Natl Ctr Cell Sci, Pune 411007, Maharashtra, India
[3] Indian Council Med Res, WHO Collaborat Ctr Diag Res Reference & Training, Reg Med Res Ctr, Port Blair 744101, Andaman & Nicob, India
[4] Tamil Nadu Vet & Anim Sci Univ, Madras 600007, Tamil Nadu, India
来源：
BRAZILIAN JOURNAL OF MICROBIOLOGY | 2010年 / 41卷 / 01期
关键词：
Leptospirosis; Leptospira borgpetersenii; lipL32; 16S rRNA; DIFFERENTIATION;
D O I：
10.1590/S1517-83822010000100022
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The main goal of this study was to evaluate the prevalence of leptospirosis among field rodents of Tiruchirappalli district, Tamil Nadu, India. In total 35 field rats were trapped and tested for seroprevalence by the microscopic agglutination test (MAT). Isolation of leptospires was performed from blood and kidney tissues and characterized to serovar level. Genomospecies identification was carried out using 16S rRNA and lipL32 gene sequencing. The molecular phylogeny was constructed to find out species segregation. Seroprevalence was about 51.4%, and the predominant serovars were Autumnalis, Javanica, Icterohaemorrhagiae and Pomona. Two isolates from the kidneys were identified as serovar Javanica of Serogroup Javanica, and sequence based molecular phylogeny indicated these two isolates were Leptospira borgpetersenii.
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收藏
页码：150 / 157
页数：8
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