CHARACTERIZATION OF LEPTOSPIRA BORGPETERSENII ISOLATES FROM FIELD RATS (RATTUS NORVEGICUS) BY 16S RRNA AND LIPL32 GENE SEQUENCING

被引：0
|
作者：
Vedhagiri, Kumaresan [1 ]
Natarajaseenivasan, Kalimuthusamy [1 ]
Prabhakaran, Shanmugarajan G. [1 ]
Selvin, Joseph [1 ]
Narayanan, Ramasamy [1 ]
Shouche, Yogesh S. [2 ]
Vijayachari, Paluru [3 ]
Ratnam, Sivalingam [4 ]
机构：
[1] Bharathidasan Univ, Sch Life Sci, Dept Microbiol, Div Med Microbiol, Tiruchirappalli 620024, Tamil Nadu, India
[2] Natl Ctr Cell Sci, Pune 411007, Maharashtra, India
[3] Indian Council Med Res, WHO Collaborat Ctr Diag Res Reference & Training, Reg Med Res Ctr, Port Blair 744101, Andaman & Nicob, India
[4] Tamil Nadu Vet & Anim Sci Univ, Madras 600007, Tamil Nadu, India
来源：
BRAZILIAN JOURNAL OF MICROBIOLOGY | 2010年 / 41卷 / 01期
关键词：
Leptospirosis; Leptospira borgpetersenii; lipL32; 16S rRNA; DIFFERENTIATION;
D O I：
10.1590/S1517-83822010000100022
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The main goal of this study was to evaluate the prevalence of leptospirosis among field rodents of Tiruchirappalli district, Tamil Nadu, India. In total 35 field rats were trapped and tested for seroprevalence by the microscopic agglutination test (MAT). Isolation of leptospires was performed from blood and kidney tissues and characterized to serovar level. Genomospecies identification was carried out using 16S rRNA and lipL32 gene sequencing. The molecular phylogeny was constructed to find out species segregation. Seroprevalence was about 51.4%, and the predominant serovars were Autumnalis, Javanica, Icterohaemorrhagiae and Pomona. Two isolates from the kidneys were identified as serovar Javanica of Serogroup Javanica, and sequence based molecular phylogeny indicated these two isolates were Leptospira borgpetersenii.
引用
收藏
页码：150 / 157
页数：8
相关论文
共 50 条
  • [21] DETECTION OF HELICOBACTER PYLORI 16S RRNA GENE IN THE YEAST ISOLATES FROM PATIENTS WITH DENTURE
    Siavoshi, F.
    Sadeghi, S.
    Beitollahi, J. M.
    Nouri, F.
    Saniee, P.
    Taghikhani, A.
    HELICOBACTER, 2012, 17 : 89 - 89
  • [22] First characterization of the intestinal microbiota in healthy Tunisian adults using 16S rRNA gene sequencing
    Khachroub, Ahlem Mahjoub
    Monnoye, Magali
    Bouhlel, Nour Elhouda
    Azaiez, Sana
    Ben Fredj, Maha
    Mansour, Wejdene
    Gerard, Philippe
    FEMS MICROBIOLOGY LETTERS, 2023, 370
  • [23] Characterization of the gut microbiota in Chinese children with overweight and obesity using 16S rRNA gene sequencing
    Chen, Xiaowei
    Zhang, Dawei
    Sun, Haixiang
    Jiang, Fei
    Shen, Yan
    Wei, Pingmin
    Shen, Xiaobing
    PEERJ, 2021, 9
  • [24] Characterization of Lung Microbiome in Subclinical Pneumonic Thai Pigs Using 16S rRNA Gene Sequencing
    Tadee, Phacharaporn
    Khaodang, Pakasinee
    Patchanee, Prapas
    Buddhasiri, Songphon
    Eiamsam-ang, Thanaporn
    Kittiwan, Nattinee
    Tadee, Pakpoom
    ANIMALS, 2025, 15 (03):
  • [25] COMPARING BACTERIAL COMMUNITIES INFERRED FROM 16S rRNA GENE SEQUENCING AND SHOTGUN METAGENOMICS
    Shah, Neethu
    Tang, Haitu
    Doak, Thomas G.
    Ye, Yuzhen
    PACIFIC SYMPOSIUM ON BIOCOMPUTING 2011, 2011, : 165 - 176
  • [26] Amplicon of 16S rRNA Gene Sequencing of Fertilized Volcanic Soils from Southern Chile
    Vargas, Rodrigo A.
    Valdes, Natalia
    Balic, Ivan
    Contreras, Eduardo, I
    Venegas, Carlos
    Aranada, Carlos P.
    Tello, Mario
    Gonzalez, Alex R.
    MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 2021, 10 (12):
  • [27] 16S rRNA gene amplicon sequencing data from an Australian wastewater treatment plant
    Romanis, C. S.
    Timms, V. J.
    Crosbie, N. D.
    Neilan, B. A.
    MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 2024, 13 (06):
  • [28] Bacterial characterization of Beijing drinking water by flow cytometry and MiSeq sequencing of the 16S rRNA gene
    Liu, Tingting
    Kong, Weiwen
    Chen, Nan
    Zhu, Jing
    Wang, Jingqi
    He, Xiaoqing
    Jin, Yi
    ECOLOGY AND EVOLUTION, 2016, 6 (04): : 923 - 934
  • [29] DNA sequencing reveals limited heterogeneity in the 16S rRNA gene from the rrnB operon among five Mycoplasma hominis isolates
    Mygind, T
    Birkelund, S
    Christiansen, G
    INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 : 1067 - 1071
  • [30] Identification of Atypical Rhodococcus-Like Clinical Isolates as Dietzia spp. by 16S rRNA Gene Sequencing
    Pilares, Lilian
    Aguero, Jesus
    Vazquez-Boland, Jose A.
    Martinez-Martinez, Luis
    Navas, Jesus
    JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (05) : 1904 - 1907
12345