Chip-Based Free-Flow Electrophoresis with Integrated Nanospray Mass-Spectrometry

被引：55
|
作者：
Benz, Christian [1 ,3 ]
Boomhoff, Michael [2 ]
Appun, Johannes [2 ]
Schneider, Christoph [2 ]
Belder, Detlev [1 ]
机构：
[1] Univ Leipzig, Inst Analyt Chem, D-04103 Leipzig, Germany
[2] Univ Leipzig, Inst Organ Chem, D-04103 Leipzig, Germany
[3] Univ Leipzig, LIFE Leipzig Res Ctr Civilizat Dis, D-04109 Leipzig, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 09期
关键词：
cycloaddition; continuous micro flow-through; electrophoresis; mass spectrometry; microfluidics; ON-A-CHIP; ZONE ELECTROPHORESIS; MICROFLUIDICS; MICROREACTORS; ELECTROSPRAY; CHEMISTRY; MICROCHIP; ONLINE; TECHNOLOGY; REACTORS;
D O I：
10.1002/anie.201409663
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Free-flow electrophoresis is an ideal tool for preparative separations in continuous microflow. With the approach presented herein for coupling free-flow electrophoresis and mass spectrometry it is now also possible to trace non-fluorescent compounds and identify them by means of mass spectrometry. The functionality of the method and its potential as an integrated separation unit for microflow synthesis is demonstrated by application to a multicomponent [3+2]-cycloannulation.
引用
收藏
页码：2766 / 2770
页数：5
相关论文
共 50 条
  • [41] A novel nanospray capillary zone electrophoresis mass spectrometry interface
    Hsieh, F
    Baronas, E
    Muir, C
    Martin, SA
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1999, 13 (01) : 67 - 72
  • [42] Integrated potentiometric detector for use in chip-based flow cells
    Tantra, R
    Manz, A
    ANALYTICAL CHEMISTRY, 2000, 72 (13) : 2875 - 2878
  • [43] PDMS free-flow electrophoresis chips with integrated partitioning bars for bubble segregation
    Koehler, Stefan
    Weilbeer, Claudia
    Howitz, Steffen
    Becker, Holger
    Beushausen, Volker
    Belder, Detlev
    LAB ON A CHIP, 2011, 11 (02) : 309 - 314
  • [44] CHARACTERIZATION OF ARTIFICIAL LIPOSOMES WITH FREE-FLOW ELECTROPHORESIS
    KESSLER, R
    MANZ, HJ
    ELECTROPHORESIS, 1990, 11 (11) : 979 - 980
  • [45] Micro free-flow electrophoresis: theory and applications
    Turgeon, Ryan T.
    Bowser, Michael T.
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2009, 394 (01) : 187 - 198
  • [46] FREE-FLOW ELECTROPHORESIS IN SOLUTIONS OF BORAX AND MANNITOL
    SHUKUN, SA
    GAVRYUSHKIN, AV
    BREZGUNOV, VN
    ZAVYALOV, VP
    ELECTROPHORESIS, 1986, 7 (12) : 572 - 574
  • [47] Reducing pH Gradients in Free-Flow Electrophoresis
    Agostino, Fletcher J.
    Cherney, Leonid T.
    Kanoatov, Mirzo
    Krylov, Sergey N.
    ANALYTICAL CHEMISTRY, 2014, 86 (12) : 5656 - 5660
  • [48] Chip-Based Capillary Zone Electrophoresis Mass Spectrometry for Rapid Resolution and Quantitation of Critical Quality Attributes in Protein Biotherapeutics
    Dykstra, Andrew B.
    Flick, Tawnya G.
    Lee, Burton
    Blue, Laura E.
    Angell, Nic
    JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2021, 32 (08) : 1952 - 1963
  • [49] SEPARATION OF HUMAN LYMPHOCYTES BY FREE-FLOW ELECTROPHORESIS
    STEIN, G
    FLAD, HD
    PABST, R
    TREPEL, F
    BIOMEDICINE EXPRESS, 1973, 19 (09): : 388 - 391
  • [50] PURIFICATION OF MONOCLONAL IGGS BY FREE-FLOW ELECTROPHORESIS
    OSTREM, JA
    MARQUEZ, R
    RHODES, B
    KOSECKI, R
    BARSTOW, L
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1990, 199 : 135 - IAEC
12345