Chip-Based Free-Flow Electrophoresis with Integrated Nanospray Mass-Spectrometry

被引：55
|
作者：
Benz, Christian [1 ,3 ]
Boomhoff, Michael [2 ]
Appun, Johannes [2 ]
Schneider, Christoph [2 ]
Belder, Detlev [1 ]
机构：
[1] Univ Leipzig, Inst Analyt Chem, D-04103 Leipzig, Germany
[2] Univ Leipzig, Inst Organ Chem, D-04103 Leipzig, Germany
[3] Univ Leipzig, LIFE Leipzig Res Ctr Civilizat Dis, D-04109 Leipzig, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 09期
关键词：
cycloaddition; continuous micro flow-through; electrophoresis; mass spectrometry; microfluidics; ON-A-CHIP; ZONE ELECTROPHORESIS; MICROFLUIDICS; MICROREACTORS; ELECTROSPRAY; CHEMISTRY; MICROCHIP; ONLINE; TECHNOLOGY; REACTORS;
D O I：
10.1002/anie.201409663
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Free-flow electrophoresis is an ideal tool for preparative separations in continuous microflow. With the approach presented herein for coupling free-flow electrophoresis and mass spectrometry it is now also possible to trace non-fluorescent compounds and identify them by means of mass spectrometry. The functionality of the method and its potential as an integrated separation unit for microflow synthesis is demonstrated by application to a multicomponent [3+2]-cycloannulation.
引用
收藏
页码：2766 / 2770
页数：5
相关论文
共 50 条
  • [31] Negative ion 'chip-based' nanospray tandem mass spectrometry for the analysis of flavonoids in glandular trichomes of Lychnophora ericoides Mart. (Asteraceae)
    Gobbo-Neto, Leonardo
    Gates, Paul J.
    Lopes, Norberto P.
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2008, 22 (23) : 3802 - 3808
  • [32] CONCENTRATION PROFILES IN FREE-FLOW ELECTROPHORESIS
    REIS, JFG
    LIGHTFOOT, EN
    LEE, H
    AICHE JOURNAL, 1974, 20 (02) : 362 - 368
  • [33] Antibody enhancement of free-flow electrophoresis
    Cohly, H.H.P.
    Morrison, D.R.
    Atassi, M.Z.
    NASA Technical Memorandum, 1988, 1 (4069): : 227 - 234
  • [34] Direct detection of additives and degradation products from polymers by liquid extraction surface analysis employing chip-based nanospray mass spectrometry
    Paine, Martin R. L.
    Barker, Philip J.
    Maclauglin, Shane A.
    Mitchell, Todd W.
    Blanksby, Stephen J.
    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2012, 26 (04) : 412 - 418
  • [35] Reprint of: Chip-based nanoelectrospray mass spectrometry of brain gangliosides
    Flangea, Corina
    Serb, Alina
    Sisu, Eugen
    Zamfir, Alina D.
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2011, 1811 (11): : 897 - 917
  • [36] CAPILLARY ELECTROPHORESIS MASS-SPECTROMETRY
    NIESSEN, WMA
    TJADEN, UR
    VANDERGREEF, J
    JOURNAL OF CHROMATOGRAPHY, 1993, 636 (01): : 3 - 19
  • [37] CAPILLARY ELECTROPHORESIS MASS-SPECTROMETRY
    CAI, JY
    HENION, J
    JOURNAL OF CHROMATOGRAPHY A, 1995, 703 (1-2) : 667 - 692
  • [38] Microfluidic Free-Flow Electrophoresis Based Solvent Exchanger for Continuously Operating Lab-on-Chip Applications
    Zitzmann, Franziska D.
    Jahnke, Heinz-Georg
    Pfeiffer, Simon A.
    Frank, Ronny
    Nitschke, Felix
    Mauritz, Laura
    Abel, Bernd
    Belder, Detlev
    Robitzki, Andrea A.
    ANALYTICAL CHEMISTRY, 2017, 89 (24) : 13550 - 13558
  • [39] Free-flow electrophoresis for top-down proteomics by Fourier transform ion cyclotron resonance mass spectrometry
    Ouvry-Patat, Severine A.
    Torres, Matthew P.
    Quek, Hung-Hiang
    Gelfand, Craig A.
    O'Mullan, Patrick
    Nissum, Mikkel
    Schroeder, Gottfried K.
    Han, Jun
    Elliott, Monica
    Dryhurst, Deanna
    Ausio, Juan
    Wolfenden, Richard
    Borchers, Christoph H.
    PROTEOMICS, 2008, 8 (14) : 2798 - 2808
  • [40] Rapid fingerprinting of a highly glycosylated fusion protein by microfluidic chip-based capillary electrophoresis-mass spectrometry
    Deyanova, Ekaterina G.
    Huang, Richard Y. -C.
    Madia, Priyanka A.
    Nandi, Pradyot
    Gudmundsson, Olafur
    Chen, Guodong
    ELECTROPHORESIS, 2021, 42 (04) : 460 - 464
12345