Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

被引：12
|
作者：
Nylk, J. [1 ,2 ]
Kristensen, M. V. G. [1 ]
Mazilu, M. [1 ]
Thayil, A. K. [1 ,2 ]
Mitchell, C. A. [1 ,2 ]
Campbell, E. C. [2 ]
Powis, S. J. [3 ]
Gunn-Moore, F. J. [2 ]
Dholakia, K. [1 ]
机构：
[1] Univ St Andrews, Sch Phys & Astron, SUPA, St Andrews KY16 9SS, Fife, Scotland
[2] Univ St Andrews, Sch Biol, St Andrews KY16 9TF, Fife, Scotland
[3] Univ St Andrews, Sch Med, St Andrews KY16 9TF, Fife, Scotland
来源：
BIOMEDICAL OPTICS EXPRESS | 2015年 / 6卷 / 04期
基金：
英国工程与自然科学研究理事会;
关键词：
TWEEZERS; MICROSCOPY; CELL; MANIPULATION; SYNAPSES;
D O I：
10.1364/BOE.6.001512
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200 mu m from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation. (C) 2015 Optical Society of America
引用
收藏
页码：1512 / 1519
页数：8
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