Identification, functional characterization, and estrogen regulation on gonadotropin-releasing hormone in the spotted scat, Scatophagus argus

被引:17
|
作者
Chen, Hua-pu [1 ]
Cui, Xue-fan [1 ]
Wang, Yao-rong [1 ]
Li, Zhi-yuan [1 ]
Tian, Chang-xu [1 ]
Jiang, Dong-neng [1 ]
Zhu, Chun-Hua [1 ]
Zhang, Yong [2 ,3 ]
Li, Shui-sheng [2 ,3 ]
Li, Guang-li [1 ]
机构
[1] Guangdong Ocean Univ, Fisheries Coll, Guangdong Res Ctr Reprod Control & Breeding Techn, Southern Marine Sci & Engn Guangdong Lab Zhanjian, Zhanjiang, Peoples R China
[2] Sun Yat Sen Univ, State Key Lab Biocontrol, Guangzhou, Peoples R China
[3] Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou, Peoples R China
基金
中国国家自然科学基金; 国家重点研发计划;
关键词
Scatophagus argus; Gonadotropin-releasing hormone; 17; beta-Estradiol; Reproduction; Feedback regulation; GENE-EXPRESSION; RAINBOW-TROUT; MOLECULAR CHARACTERIZATION; GILTHEAD SEABREAM; SEXUAL STEROIDS; GNRH SYSTEM; ER-ALPHA; FORMS; BRAIN; PITUITARY;
D O I
10.1007/s10695-020-00825-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Gonadotropin-releasing hormone (GnRH) is a key neuropeptide of the reproductive system. However, little is known about the role of GnRH in the spotted scat (Scatophagus argus). Here, three GnRH subtypes (cGnRH-II, sGnRH, and sbGnRH) were identified in the spotted scat. cGnRH-II and sGnRH were only expressed in the brains and gonads of both male and female fish, exhibiting a tissue-specific expression pattern, while sbGnRH was expressed at different transcription levels in all examined tissues. During ovarian maturation, hypothalamus-associated sbGnRH was upregulated, while the expression of sGnRH was variable and cGnRH-II first increased and then decreased. In vivo experiments showed that sbGnRH significantly promoted the expression of fsh and lh genes in a dose-dependent manner and exhibited a desensitization effect on lh expression at high concentrations. For sGnRH and cGnRH-II, only high concentrations could induce fsh and lh expression. Furthermore, treatment with highly concentrated sbGnRH peptide also induced fsh and lh expression, whereas the sGnRH and cGnRH-II peptides only induced fsh expression in vitro. 17 beta-Estradiol (E-2) significantly inhibited the expression of sbGnRH mRNA in a dose-dependent manner and did not impact sGnRH and cGnRH-II mRNA levels in vivo or in vitro. The inhibitory effect of E-2 on sbGnRH expression was attenuated by the estrogen receptor (ER) broad-spectrum antagonist (fulvestrant) and the ER alpha-specific antagonist (methyl-piperidinopyrazole), respectively, implying that the feedback regulation on sbGnRH is mediated via ER alpha. This study provides a theoretical basis for the reproductive endocrinology of the spotted scat by studying GnRH.
引用
收藏
页码:1743 / 1757
页数:15
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