Identification, functional characterization, and estrogen regulation on gonadotropin-releasing hormone in the spotted scat, Scatophagus argus

被引:17
|
作者
Chen, Hua-pu [1 ]
Cui, Xue-fan [1 ]
Wang, Yao-rong [1 ]
Li, Zhi-yuan [1 ]
Tian, Chang-xu [1 ]
Jiang, Dong-neng [1 ]
Zhu, Chun-Hua [1 ]
Zhang, Yong [2 ,3 ]
Li, Shui-sheng [2 ,3 ]
Li, Guang-li [1 ]
机构
[1] Guangdong Ocean Univ, Fisheries Coll, Guangdong Res Ctr Reprod Control & Breeding Techn, Southern Marine Sci & Engn Guangdong Lab Zhanjian, Zhanjiang, Peoples R China
[2] Sun Yat Sen Univ, State Key Lab Biocontrol, Guangzhou, Peoples R China
[3] Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou, Peoples R China
基金
中国国家自然科学基金; 国家重点研发计划;
关键词
Scatophagus argus; Gonadotropin-releasing hormone; 17; beta-Estradiol; Reproduction; Feedback regulation; GENE-EXPRESSION; RAINBOW-TROUT; MOLECULAR CHARACTERIZATION; GILTHEAD SEABREAM; SEXUAL STEROIDS; GNRH SYSTEM; ER-ALPHA; FORMS; BRAIN; PITUITARY;
D O I
10.1007/s10695-020-00825-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Gonadotropin-releasing hormone (GnRH) is a key neuropeptide of the reproductive system. However, little is known about the role of GnRH in the spotted scat (Scatophagus argus). Here, three GnRH subtypes (cGnRH-II, sGnRH, and sbGnRH) were identified in the spotted scat. cGnRH-II and sGnRH were only expressed in the brains and gonads of both male and female fish, exhibiting a tissue-specific expression pattern, while sbGnRH was expressed at different transcription levels in all examined tissues. During ovarian maturation, hypothalamus-associated sbGnRH was upregulated, while the expression of sGnRH was variable and cGnRH-II first increased and then decreased. In vivo experiments showed that sbGnRH significantly promoted the expression of fsh and lh genes in a dose-dependent manner and exhibited a desensitization effect on lh expression at high concentrations. For sGnRH and cGnRH-II, only high concentrations could induce fsh and lh expression. Furthermore, treatment with highly concentrated sbGnRH peptide also induced fsh and lh expression, whereas the sGnRH and cGnRH-II peptides only induced fsh expression in vitro. 17 beta-Estradiol (E-2) significantly inhibited the expression of sbGnRH mRNA in a dose-dependent manner and did not impact sGnRH and cGnRH-II mRNA levels in vivo or in vitro. The inhibitory effect of E-2 on sbGnRH expression was attenuated by the estrogen receptor (ER) broad-spectrum antagonist (fulvestrant) and the ER alpha-specific antagonist (methyl-piperidinopyrazole), respectively, implying that the feedback regulation on sbGnRH is mediated via ER alpha. This study provides a theoretical basis for the reproductive endocrinology of the spotted scat by studying GnRH.
引用
收藏
页码:1743 / 1757
页数:15
相关论文
共 50 条
  • [31] Regulation of gonadotropin-releasing hormone gene transcription
    Chandran, UR
    DeFranco, DB
    BEHAVIOURAL BRAIN RESEARCH, 1999, 105 (01) : 29 - 36
  • [32] Cloning, expression and functional characterization on vitellogenesis of estrogen receptors in Scatophagus argus
    Cui, Xue-Fan
    Zhao, Yuan
    Chen, Hua-Pu
    Deng, Si-Ping
    Jiang, Dong-Neng
    Wu, Tian-Li
    Zhu, Chun-Hua
    Li, Guang-Li
    GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2017, 246 : 37 - 45
  • [33] Behavioral regulation of gonadotropin-releasing hormone production
    Rissman, EF
    Li, X
    King, JA
    Millar, RP
    BRAIN RESEARCH BULLETIN, 1997, 44 (04) : 459 - 464
  • [34] Functions of thyroid hormone signaling in regulating melanophore, iridophore, erythrophore, and pigment pattern formation in spotted scat (Scatophagus argus)
    Liao, Yongguan
    Han, Tong
    Jiang, Dongneng
    Zhu, Chunhua
    Shi, Gang
    Li, Guangli
    Shi, Hongjuan
    BMC GENOMICS, 2025, 26 (01):
  • [35] Regulation of gonadotropin-releasing hormone in nonhypothalamic tissues
    Kim, Helen H.
    Mui, Keeley L.
    Nikrodhanond, Amisra A.
    Tamayo, Nina Carmelo R.
    SEMINARS IN REPRODUCTIVE MEDICINE, 2007, 25 (05) : 326 - 336
  • [36] REGULATION OF GONADOTROPIN-RELEASING HORMONE RECEPTORS INVITRO
    LOUMAYE, E
    CATT, K
    CLINICAL RESEARCH, 1982, 30 (02): : A491 - A491
  • [37] FUNCTIONAL GONADOTROPIN-RELEASING HORMONE DEFICIENCY IN MEN
    Lewkowitz-Shpuntoff, Hilana
    Balasubramanian, Ravikumar
    Chavan, Niraj
    Kaina, Jennifer
    Dwyer, Andrew
    Seminara, Stephanie
    Plummer, Lacey
    Hayes, Francis
    Crowley, William F., Jr.
    Pitteloud, Nelly
    JOURNAL OF INVESTIGATIVE MEDICINE, 2012, 60 (03) : 619 - 619
  • [38] Molecular cloning, tissue distribution, and pharmacological characterization of melanocortin-4 receptor in spotted scat, Scatophagus argus
    Li, Jian-Tao
    Yang, Zhao
    Chen, Hua-Pu
    Zhu, Chun-Hua
    Deng, Si-Ping
    Li, Guang-Li
    Tao, Ya-Xiong
    GENERAL AND COMPARATIVE ENDOCRINOLOGY, 2016, 230 : 143 - 152
  • [39] IDENTIFICATION AND CHARACTERIZATION OF THE RADIOIODINATION PRODUCTS OF GONADOTROPIN-RELEASING HORMONE .1.
    MOCTEZUMA, O
    VILLANUEVA, C
    GARZAFLORES, J
    OLIART, RM
    LARREA, F
    REVISTA DE INVESTIGACION CLINICA-CLINICAL AND TRANSLATIONAL INVESTIGATION, 1984, 36 (01): : 31 - 38
  • [40] CHARACTERIZATION OF AN ANTIBODY TO LAMPREY GONADOTROPIN-RELEASING HORMONE
    CALVIN, JL
    LAUDANO, AP
    SOWER, SA
    AMERICAN ZOOLOGIST, 1989, 29 (04): : A94 - A94