α-Glucosidase Inhibitory Mechanism of Phloridzin

被引：0

作者：

Liu, Jiangwei ^{[1
]}

Chen, Yuzhen ^{[2
]}

Liu, Benguo ^{[1
]}

Liang, Guizhao ^{[3
]}

机构：

[1] Henan Inst Sci & Technol, Sch Food Sci, Xinxiang 453003, Peoples R China

[2] Henan Inst Sci & Technol, Sch Math Sci, Xinxiang 453003, Peoples R China

[3] Chongqing Univ, Sch Bioengn, Key Lab Biorheol Sci & Technol, Minist Educ, Chongqing 400044, Peoples R China

来源：

JOURNAL OF THE CHEMICAL SOCIETY OF PAKISTAN | 2018年 / 40卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Phloridzin; alpha-Glucosidase; Inhibitory mechanism; Fluorescence spectroscopy; Molecular docking; MOLECULAR DOCKING; FLAVONOIDS; IDENTIFICATION; ANTIOXIDANT; DERIVATIVES; AMYLASE; 3D-QSAR; TRYPSIN; SEARCH;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Phloridzin is a chalcone with potential application in functional food and medicine. In this study, the alpha-glucosidase inhibitory mechanism of phloridzin was investigated by inhibitory test, fluorescence spectroscopy and molecular docking method. The IC50 value of phloridzin was determined at 0.68 mmol/L. With the increase of phloridzin concentration, the static fluorescence quenching of alpha-glucosidase could be observed. The quenching constant (Kq), binding constant (K-A) and the number of binding site (n) of phloridzin to alpha-glucosidase were 6.1474x10(12), 1.3243X10(5) and 1.076, respectively. The molecular docking result suggested that phloridzin could play the inhibitory role by binding the Lys510 and G1u374 of alpha-glucosidase with hydrogen bonds.

引用

页码：799 / 803

页数：5

共 50 条

[31] Inhibitory effect of corosolic acid on α-glucosidase: kinetics, interaction mechanism, and molecular simulation
Ni, Mengting
Pan, Junhui
Hu, Xing
Gong, Deming
Zhang, Guowen
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2019, 99 (13) : 5881 - 5889
[32] Inhibitory mechanism of two allosteric inhibitors, oleanolic acid and ursolic acid on α-glucosidase
Ding, Huafang
Hu, Xing
Xu, Ximing
Zhang, Guowen
Gong, Deming
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 107 : 1844 - 1855
[33] Molecular dynamics simulations reveal the inhibitory mechanism of Withanolide A against α-glucosidase and α-amylase
Oyewusi, Habeebat Adekilekun
Wu, Yuan-Seng
Safi, Sher Zaman
Wahab, Roswanira Abdul
Hatta, Mohd Hayrie Mohd
Batumalaie, Kalaivani
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2023, 41 (13): : 6203 - 6218
[34] The masking mechanism of catechin to the sweet taste of phloridzin
Wei, Fang
Luo, Liyong
Wang, Xi
Luo, Wei
Wu, Fan
Tian, Shiyi
Qin, Yumei
Zeng, Liang
FOOD CHEMISTRY, 2025, 464
[35] On the mechanism of milk secretion - The influence of insulin and phloridzin
Gowen, JW
Tobey, ER
JOURNAL OF GENERAL PHYSIOLOGY, 1931, 15 (01): : 67 - 85
[36] Inhibitory Effect of Genkwanin on α-Glucosidase
Jiang Y.
Chen Q.
Zhang L.
Gao X.
Science and Technology of Food Industry, 2021, 42 (15) : 43 - 47
[37] Novel Insights into the Inhibitory Effect and Mechanism of Proanthocyanidins from Pyracantha fortuneana Fruit on α-Glucosidase
Wei, Mankun
Chai, Wei-Ming
Yang, Qin
Wang, Rui
Peng, Yiyuan
JOURNAL OF FOOD SCIENCE, 2017, 82 (10) : 2260 - 2268
[38] Inhibitory kinetics and mechanism of rifampicin on α-glucosidase: Insights from spectroscopic and molecular docking analyses
Lin, Mei-Zhen
Chai, Wei-Ming
Zheng, Yun-Ling
Huang, Qian
Ou-Yang, Chong
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 122 : 1244 - 1252
[39] Structural changes and molecular mechanism study on the inhibitory activity of epigallocatechin against α-glucosidase and α-amylase
Man, Ziyi
Feng, Yi
Xiao, Jibo
Yang, Hailong
Wu, Xiangting
FRONTIERS IN NUTRITION, 2022, 9
[40] Inhibitory effects of phloridzin dihydrate on the activity of mushroom (Agaricus hisporus) tyrosinase
Wan, Qin
Qiu, Ling
Chen, Xiang-ren
Song, Kang-Kang
Shi, Yan
Chen, Qing-Xi
BIOORGANIC & MEDICINAL CHEMISTRY, 2007, 15 (03) : 1568 - 1571

← 1 2 3 4 5 →