Rapid coliphage detection assay

被引:8
|
作者
Stanek, JE [1 ]
Falkinham, JO [1 ]
机构
[1] Virginia Polytech Inst & State Univ, Dept Biol, Blacksburg, VA 24061 USA
关键词
Escherichia coli; beta-galactosidase; coliphage;
D O I
10.1016/S0166-0934(00)00253-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A rapid coliphage detection assay was developed, based on the phage-induced release of beta -galactosidase from cells of Escherichia coli. The assay could detect as few as five coliphage per sample without an overnight incubation period. The range of acceptable assay parameters was identified. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:93 / 98
页数:6
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