Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: Factors regulating the protein release

被引：115

作者：

Balabushevitch, NG

Sukhorukov, GB ^{[1
]}

Moroz, NA

Volodkin, DV

Larionova, NI

Donath, E

Mohwald, H

机构：

[1] Max Planck Inst Colloids & Interfaces, D-14476 Golm, Germany

[2] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119899, Russia

[3] Russian Acad Sci, Inst Crystallog, Moscow 117333, Russia

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2001年 / 76卷 / 03期

关键词：

protein encapsulation; polyelectrolytes; ultrathin organic films; protein aggregates; microcapsules; colloid; protein release;

D O I：

10.1002/bit.1184

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel method of protein encapsulation is proposed. Preformed protein aggregates are covered with polyelectrolyte layers by means of layer-by-layer adsorption. The polyelectrolyte membrane prevents protein leakage out of the capsule. Using chymotrypsin as a model enzyme the capsule wall selective permeability was demonstrated for substrates and inhibitors of different molecular weight and solubility. (C) 2001 John Wiley & Sons, Inc.

引用

页码：207 / 213

页数：7

共 50 条

[41] Time Controlled Protein Release from Layer-by-Layer Assembled Multilayer Functionalized Agarose Hydrogels
Mehrotra, Sumit
Lynam, Daniel
Maloney, Ryan
Pawelec, Kendell M.
Tuszynski, Mark H.
Lee, Ilsoon
Chan, Christina
Sakamoto, Jeffrey
ADVANCED FUNCTIONAL MATERIALS, 2010, 20 (02) : 247 - 258
[42] Enhanced viability of Lactiplantibacillus plantarun by encapsulation with whey protein isolate fibrils and polysaccharides through layer-by-layer coating
Li, Siyuan
Su, Wei
Zhang, Yu
Gan, Wanling
Liu, Xiaoli
Fan, Linlin
FOOD HYDROCOLLOIDS, 2025, 163
[43] Assembly of glucose oxidase and different polyelectrolytes by means of electrostatic layer-by-layer adsorption on thiolated gold surface
Rodríguez, MC
Rivas, GA
ELECTROANALYSIS, 2004, 16 (20) : 1717 - 1722
[44] Layer-by-Layer self-assembly of polyaspartate and Poly(ethyleneimine) on magnetic nanoparticles: Characterization and adsorption of protein
Wang, Shixing
Zhou, Yang
Niu, Hua
Zhang, Xuezhong
CURRENT APPLIED PHYSICS, 2011, 11 (06) : 1337 - 1342
[45] Rational design of a degradable polyanion for layer-by-layer assembly for encapsulation and release of cationic functional biomolecules
Hiraoka, Ryuichi
Funasaki, Yuichi
Ishii, Jun
Maruyama, Tatsuo
CHEMICAL COMMUNICATIONS, 2015, 51 (98) : 17447 - 17450
[46] Graphene Oxide Influence the Release of Protein from Layer-by-Layer Self Assembled Electrospun PCL Fiber
Kolanthai, E.
Catalani, L. H.
TISSUE ENGINEERING PART A, 2017, 23 : S25 - S25
[47] Layer-by-layer method for immobilization of protein molecules on biochip surface
Zhavnerko, GK
Yi, SJ
Kweon, SM
Ha, KS
FRONTIERS OF MULTIFUNCTIONAL NANOSYSTEMS, 2002, 57 : 79 - 90
[48] Protein Induces Layer-by-Layer Exfoliation of Transition Metal Dichalcogenides
Guan, Guijian
Zhang, Shuangyuan
Liu, Shuhua
Cai, Yongqing
Low, Michelle
Teng, Choon Peng
Phang, In Yee
Cheng, Yuan
Duei, Koh Leng
Srinivasan, Bharathi Madurai
Zheng, Yuangang
Zhang, Yong-Wei
Han, Ming-Yong
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (19) : 6152 - 6155
[49] Layer-by-layer of liposomes and membrane protein as a recognition element of biosensor
Nakane, Yuko
Kubo, Izumi
THIN SOLID FILMS, 2009, 518 (02) : 678 - 681
[50] LAYER-BY-LAYER ASSEMBLY OF ALTERNATE PROTEIN POLYION ULTRATHIN FILMS
LVOV, Y
ARIGA, K
KUNITAKE, T
CHEMISTRY LETTERS, 1994, (12) : 2323 - 2326

← 1 2 3 4 5 →