Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: Factors regulating the protein release

被引：115
|
作者：
Balabushevitch, NG
Sukhorukov, GB [1 ]
Moroz, NA
Volodkin, DV
Larionova, NI
Donath, E
Mohwald, H
机构：
[1] Max Planck Inst Colloids & Interfaces, D-14476 Golm, Germany
[2] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119899, Russia
[3] Russian Acad Sci, Inst Crystallog, Moscow 117333, Russia
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 2001年 / 76卷 / 03期
关键词：
protein encapsulation; polyelectrolytes; ultrathin organic films; protein aggregates; microcapsules; colloid; protein release;
D O I：
10.1002/bit.1184
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A novel method of protein encapsulation is proposed. Preformed protein aggregates are covered with polyelectrolyte layers by means of layer-by-layer adsorption. The polyelectrolyte membrane prevents protein leakage out of the capsule. Using chymotrypsin as a model enzyme the capsule wall selective permeability was demonstrated for substrates and inhibitors of different molecular weight and solubility. (C) 2001 John Wiley & Sons, Inc.
引用
收藏
页码：207 / 213
页数：7
相关论文
共 50 条
  • [21] Protein microparticles with controlled stability prepared via layer-by-layer adsorption of biopolyelectrolytes
    Balabushevich, N. G.
    Izumrudov, V. A.
    Larionova, N. I.
    POLYMER SCIENCE SERIES A, 2012, 54 (07) : 540 - 551
  • [22] Protein microparticles with controlled stability prepared via layer-by-layer adsorption of biopolyelectrolytes
    N. G. Balabushevich
    V. A. Izumrudov
    N. I. Larionova
    Polymer Science Series A, 2012, 54 : 540 - 551
  • [23] Layer-by-layer adsorption of weak polyelectrolytes: A means to control layer thickness, composition and surface wettability
    Shiratori, S
    Rubner, MF
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1998, 216 : U735 - U735
  • [24] Influence of different polyelectrolytes on layer-by-layer microcapsule properties: Encapsulation efficiency and colloidal and temperature stability
    Mak, Wing Cheung
    Cheung, Kwan Yee
    Trau, Dieter
    CHEMISTRY OF MATERIALS, 2008, 20 (17) : 5475 - 5484
  • [25] Mechanisms of adsorption of an azo-polyelectrolyte onto layer-by-layer films
    Ferreira, Quirina
    Gomes, Paulo J.
    Maneira, Manuel J. P.
    Ribeiro, Paulo A.
    Raposo, Maria
    SENSORS AND ACTUATORS B-CHEMICAL, 2007, 126 (01) : 311 - 317
  • [26] Layer-by-layer adsorption of biopolyelectrolytes as a universal approach to fabrication of protein-loaded microparticles
    Balabushevich N.G.
    Pechenkin M.A.
    Lopes de Gerenyu A.V.
    Zorov I.N.
    Mikhalchik E.V.
    Larionova N.I.
    Moscow University Chemistry Bulletin, 2014, 69 (3) : 117 - 124
  • [27] Modification of Magnetic Particles via Layer-by-Layer Self-Assembly and Adsorption of Protein
    Sun Wentong
    Wang Shixing
    Zhang Xiaohong
    Zhong Dubo
    Wang Yaqin
    Zhu Hongkun
    Li Junming
    ACTA CHIMICA SINICA, 2011, 69 (03) : 351 - 355
  • [28] Microcapsules for controlled release fabricated via layer-by-layer self-assembly of polyelectrolytes
    Wang, Chaoyang
    Ye, Shiqu
    Sun, Qilong
    He, Chengyi
    Ye, Weihua
    Liu, Xinxing
    Tong, Zhen
    JOURNAL OF EXPERIMENTAL NANOSCIENCE, 2008, 3 (02) : 133 - 145
  • [29] Sustained protein release from hydrogel microparticles using layer-by-layer (LbL) technology
    Sakr, Omar S.
    Jordan, Olivier
    Borchard, Gerrit
    DRUG DELIVERY, 2016, 23 (08) : 2747 - 2755
  • [30] Adsorption of protein onto double layer mixed matrix membranes
    Sun, Junfen
    Wu, Lishun
    COLLOIDS AND SURFACES B-BIOINTERFACES, 2014, 123 : 33 - 38
12345