Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: Factors regulating the protein release

被引：115
|
作者：
Balabushevitch, NG
Sukhorukov, GB [1 ]
Moroz, NA
Volodkin, DV
Larionova, NI
Donath, E
Mohwald, H
机构：
[1] Max Planck Inst Colloids & Interfaces, D-14476 Golm, Germany
[2] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119899, Russia
[3] Russian Acad Sci, Inst Crystallog, Moscow 117333, Russia
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 2001年 / 76卷 / 03期
关键词：
protein encapsulation; polyelectrolytes; ultrathin organic films; protein aggregates; microcapsules; colloid; protein release;
D O I：
10.1002/bit.1184
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A novel method of protein encapsulation is proposed. Preformed protein aggregates are covered with polyelectrolyte layers by means of layer-by-layer adsorption. The polyelectrolyte membrane prevents protein leakage out of the capsule. Using chymotrypsin as a model enzyme the capsule wall selective permeability was demonstrated for substrates and inhibitors of different molecular weight and solubility. (C) 2001 John Wiley & Sons, Inc.
引用
收藏
页码：207 / 213
页数：7
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