Molecular typing of a suspected cluster of Nocardia farcinica infections by use of randomly amplified polymorphic DNA, pulsed-field gel electrophoresis, and amplified fragment length polymorphism analyses

被引:4
|
作者
Kalpoe, J. S. [1 ]
Templeton, K. E. [1 ]
Horrevorts, A. M. [2 ]
Endtz, H. P. [3 ]
Kuijper, E. J. [1 ]
Bernards, A. T. [1 ]
Klaassen, C. H. W. [2 ]
机构
[1] Leiden Univ, Ctr Med, Dept Med Microbiol, NL-2300 RC Leiden, Netherlands
[2] Canisius Wilhelmina Hosp, Dept Med Microbiol & Infect Dis, NL-6532 SZ Nijmegen, Netherlands
[3] Univ Rotterdam Med Ctr, Erasmus Med Ctr, Dept Microbiol & Infect Dis, NL-3015 GD Rotterdam, Netherlands
关键词
D O I
10.1128/JCM.00932-07
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Randomly amplified polymorphic DNA (RAPD), pulsed-field gelelectrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) analyses were used to investigate a possible outbreak of Nocardia farcinica. RAPD and PFGE analyses yielded irreproducible and unsatisfactory results, respectively. AFLP analysis seem to be a promising and welcome addition for molecular analysis of Nocardia isolates.
引用
收藏
页码:4048 / 4050
页数:3
相关论文
共 50 条
  • [31] Amplified fragment length polymorphism and pulsed field gel electrophoresis for subspecies differentiation of Serpulina pilosicoli
    Moller, K
    Jensen, TK
    Boye, M
    Leser, TD
    Ahrens, P
    ANAEROBE, 1999, 5 (3-4) : 313 - 315
  • [32] Identification and typing of Malassezia yeasts using amplified fragment length polymorphism (AFLPTm), random amplified polymorphic DNA (RAPD) and denaturing gradient gel electrophoresis (DGGE)
    Theelen, Bart
    Silvestri, Massimiliano
    Gueho, Eveline
    van Belkum, Alex
    Boekhout, Teun
    FEMS YEAST RESEARCH, 2001, 1 (02) : 79 - 86
  • [33] COMPARISON OF RIBOTYPING, PULSED-FIELD GEL-ELECTROPHORESIS AND RANDOM AMPLIFIED POLYMORPHIC DNA FOR TYPING CLOSTRIDIUM-DIFFICILE STRAINS
    CHACHATY, E
    SAULNIER, P
    MARTIN, A
    MARIO, N
    ANDREMONT, A
    FEMS MICROBIOLOGY LETTERS, 1994, 122 (1-2) : 61 - 68
  • [34] Use of Random Amplified Polymorphic DNA Analysis and Single-Enzyme Amplified Fragment Length Polymorphism in Molecular Typing of Ornithobacterium rhinotracheale Strains
    Chou, Chung-Hsi
    Lin, Shin-Ying
    Chen, Chiou-Lin
    Tsai, Hsiang-Jung
    AVIAN DISEASES, 2009, 53 (01) : 108 - 114
  • [35] Determining the genetic structure of the natural population of Staphylococcus aureus:: A comparison of multilocus sequence typing with pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing
    Grundmann, H
    Hori, S
    Enright, MC
    Webster, C
    Tami, A
    Feil, EJ
    Pitt, T
    JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (12) : 4544 - 4546
  • [36] Molecular Epidemiology of Japanese Avian Pasteurella multocida Strains by the Single-Enzyme Amplified Fragment Length Polymorphism and Pulsed-Field Gel Electrophoresis
    Sthitmatee, Nattawooti
    Kataoka, Yasushi
    Sawada, Takuo
    JOURNAL OF VETERINARY MEDICAL SCIENCE, 2010, 72 (11): : 1465 - 1470
  • [37] Pulsed-field gel electrophoresis is more discriminating than multilocus enzyme electrophoresis and random amplified polymorphic DNA analysis for typing pyogenic streptococci
    Bert, F
    Branger, C
    LambertZechovsky, N
    CURRENT MICROBIOLOGY, 1997, 34 (04) : 226 - 229
  • [38] Characterisation of persistent and sporadic Listeria monocytogenes strains by pulsed-field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP)
    Autio, T
    Keto-Timonen, R
    Lundén, J
    Björkroth, J
    Korkeala, H
    SYSTEMATIC AND APPLIED MICROBIOLOGY, 2003, 26 (04) : 539 - 545
  • [39] Pulsed-Field Gel Electrophoresis Is More Discriminating Than Multilocus Enzyme Electrophoresis and Random Amplified Polymorphic DNA Analysis for Typing Pyogenic Streptococci
    Frédéric Bert
    Catherine Branger
    Nicole Lambert-Zechovsky
    Current Microbiology , 1997, 34 : 226 - 229
  • [40] FluoMEP: A new genotyping method combining the advantages of randomly amplified polymorphic DNA and amplified fragment length polymorphism
    Chang, Alex
    Liew, Woei Chang
    Chuah, Aaron
    Lim, Zijie
    Lin, Qifeng
    Orban, Laszlo
    ELECTROPHORESIS, 2007, 28 (04) : 525 - 534