Lithographing of biomolecules on a substrate surface using an enzyme-immobilized AFM tip

被引：48

作者：

Takeda, S

Nakamura, C

Miyamoto, C

Nakamura, N

Kageshima, M

Tokumoto, H

Miyake, J

机构：

[1] Natl Inst AIST, TERC, Amagasaki, Hyogo 6610974, Japan

[2] Tokyo Univ Agr & Technol, Dept Biotechnol, Koganei, Tokyo 1848588, Japan

[3] Natl Inst AIST, NRI, Tsukuba, Ibaraki 3058562, Japan

来源：

NANO LETTERS | 2003年 / 3卷 / 11期

关键词：

D O I：

10.1021/nl034448k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A method of enzymatic lithography was successfully performed in a buffered solution using Staphylococcal serine V8 protease and atomic force microscopy (AFM). The retained activity of the protease immobilized on the AFM tip was confirmed by force measurement to rupture the enzyme-substrate complex. After contact scanning using the enzyme-immobilized tip to the substrate peptide layer, a square shape of the lithographed area was clearly observed by subsequent AFM imaging.

引用

页码：1471 / 1474

页数：4

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