Engineering and expression of a recombinant fusion protein possessing fibroblast growth factor-2 and fibronectin fragment

被引：8
|
作者：
Jang, JH [1 ]
Chung, CP
机构：
[1] Inha Univ, Coll Med, Dept Biochem, Inchon 400712, South Korea
[2] Seoul Natl Univ, Coll Dent, Intellectual Biointerface Engn Ctr, Seoul 110768, South Korea
[3] Seoul Natl Univ, Coll Dent, Dept Periodontol, Seoul 110768, South Korea
来源：
BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 24期
关键词：
cell adhesion; extracellular matrix; fibroblast growth factor; fibronectin; fusion;
D O I：
10.1007/s10529-004-5278-1
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
There is a synergistic effect between fibronectin and fibroblast growth factor 2 (FGF-2) on osteoblast cell adhesion through an extracellular, signal-regulated kinase pathway. Here we describe the engineering of a fusion protein containing fibronectin fragment (FNIII9-10) connected to the COOH-terminus of FGF-2. Purified FGF2-FNIII9-10 fusion protein exhibited a significant increase of cell adhesion and proliferation of MG63 cells compared with FNIII9-10 alone (p < 0.05).
引用
收藏
页码：1837 / 1840
页数：4
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