Engineering and expression of a recombinant fusion protein possessing fibroblast growth factor-2 and fibronectin fragment

被引：8
|
作者：
Jang, JH [1 ]
Chung, CP
机构：
[1] Inha Univ, Coll Med, Dept Biochem, Inchon 400712, South Korea
[2] Seoul Natl Univ, Coll Dent, Intellectual Biointerface Engn Ctr, Seoul 110768, South Korea
[3] Seoul Natl Univ, Coll Dent, Dept Periodontol, Seoul 110768, South Korea
来源：
BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 24期
关键词：
cell adhesion; extracellular matrix; fibroblast growth factor; fibronectin; fusion;
D O I：
10.1007/s10529-004-5278-1
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
There is a synergistic effect between fibronectin and fibroblast growth factor 2 (FGF-2) on osteoblast cell adhesion through an extracellular, signal-regulated kinase pathway. Here we describe the engineering of a fusion protein containing fibronectin fragment (FNIII9-10) connected to the COOH-terminus of FGF-2. Purified FGF2-FNIII9-10 fusion protein exhibited a significant increase of cell adhesion and proliferation of MG63 cells compared with FNIII9-10 alone (p < 0.05).
引用
收藏
页码：1837 / 1840
页数：4
相关论文
共 50 条
  • [21] A glycopolymer chaperone for fibroblast growth factor-2
    Guan, R
    Sun, XL
    Hou, SJ
    Wu, PY
    Chaikof, EL
    BIOCONJUGATE CHEMISTRY, 2004, 15 (01) : 145 - 151
  • [22] Biological roles of fibroblast growth factor-2
    Bikfalvi, A
    Klein, S
    Pintucci, G
    Rifkin, DB
    ENDOCRINE REVIEWS, 1997, 18 (01) : 26 - 45
  • [23] The effect of fibroblast growth factor-2 on the osteoinductive activity of recombinant human bone morphogenetic protein-2 in rat muscle
    Fujimura, K
    Bessho, K
    Okubo, Y
    Kusumoto, K
    Segami, N
    Iizuka, T
    ARCHIVES OF ORAL BIOLOGY, 2002, 47 (08) : 577 - 584
  • [24] Expression of fibroblast growth factor-2 and fibroblast growth factor receptor-1 protein in the hippocampus in rats exhibiting chronic stress-induced depression
    Hou, Gonglin
    Tang, Mingming
    NEURAL REGENERATION RESEARCH, 2011, 6 (13) : 1010 - 1016
  • [25] Expression of fibroblast growth factor-2 and fibroblast growth factor receptor-1 protein in the hippocampus in rats exhibiting chronic stress-induced depression
    Gonglin Hou
    Neural Regeneration Research, 2011, 6 (13) : 1010 - 1016
  • [26] Mechanisms of fibroblast growth factor-2 modulation of vascular endothelial growth factor expression by osteoblastic cells
    Saadeh, PB
    Mehrara, BJ
    Steinbrech, DS
    Spector, JA
    Greenwald, JA
    Chim, GS
    Ueno, H
    Gittes, GK
    Longaker, MT
    ENDOCRINOLOGY, 2000, 141 (06) : 2075 - 2083
  • [27] Fibroblast growth factor-2 regulates expression of osteopontin in periodontal ligament cells
    Terashima, Yoshimitsu
    Shimabukuro, Yoshio
    Terashima, Hiroaki
    Ozasa, Masao
    Terakura, Mami
    Ikezawa, Kazuhiko
    Hashikawa, Tomoko
    Takedachi, Masahide
    Oohara, Hiroyuki
    Yamada, Satoru
    Murakami, Shinya
    JOURNAL OF CELLULAR PHYSIOLOGY, 2008, 216 (03) : 640 - 650
  • [28] Stimulatory role of dopamine on fibroblast growth factor-2 expression in rat striatum
    Roceri, M
    Molteni, R
    Fumagalli, F
    Racagni, G
    Gennarelli, M
    Corsini, GU
    Maggio, R
    Riva, MA
    JOURNAL OF NEUROCHEMISTRY, 2001, 76 (04) : 990 - 997
  • [29] Parathyroid hormone and cAMP increase the expression of fibroblast growth factor-2 and the fibroblast growth factor receptors in osteoblastic cells.
    Hurley, MM
    Abreu, C
    Tetradis, S
    Kream, BE
    Raisz, LG
    JOURNAL OF BONE AND MINERAL RESEARCH, 1996, 11 : T350 - T350
  • [30] Parathyroid hormone regulates the expression of fibroblast growth factor-2 mRNA and fibroblast growth factor receptor mRNA in osteoblastic cells
    Hurley, MM
    Tetradis, S
    Huang, YF
    Hock, J
    Kream, BE
    Raisz, LG
    Sabbieti, MG
    JOURNAL OF BONE AND MINERAL RESEARCH, 1999, 14 (05) : 776 - 783
12345