Development of a combined technique using a rapid one-step immunochromatographic assay and indirect competitive ELISA for the rapid detection of baicalin

被引:26
|
作者
Paudel, Madan Kumar [1 ]
Putalun, Waraporn [2 ]
Sritularak, Boonchoo [3 ]
Morinaga, Osamu [4 ]
Shoyama, Yukihiro [4 ]
Tanaka, Hiroyuki [1 ]
Morimoto, Satoshi [1 ]
机构
[1] Kyushu Univ, Dept Pharmacognosy, Grad Sch Pharmaceut Sci, Fukuoka 8128582, Japan
[2] Khon Kaen Univ, Fac Pharmaceut Sci, Khon Kaen 40002, Thailand
[3] Chulalongkorn Univ, Fac Pharmaceut Sci, Bangkok 10330, Thailand
[4] Nagasaki Int Univ, Fac Pharmaceut Sci, Nagasaki 8593298, Japan
关键词
Baicalin; Scutellaria Radix; Immunochromatographic strip; Anti-baicalin monoclonal antibody; Enzyme-linked immunosorbent assay; SCUTELLARIA-BAICALENSIS-GEORGI; LINKED-IMMUNOSORBENT-ASSAY; MONOCLONAL-ANTIBODY; IN-VITRO; RADIX; FLAVONOIDS; CHROMATOGRAPHY; MEDICINES; CELLS;
D O I
10.1016/j.aca.2011.05.054
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A colloidal gold conjugated anti-baicalin monoclonal antibody (anti-BA MAb) was prepared and used in an immunochromatographic assay (ICA) for BA in Scutellariae Radix and Kampo medicines. This competitive ICA uses an anti-BA MAb which shows a high specificity for BA and baicalein. Its advantages include a short assay time (15 min), no dependence on any instrumental systems, and it can detect BA in plant materials and Kampo medicines. The limit of detection for the ICA was found to be around 0.6 mu g mL(-1) of baicalin. Moreover, the usefulness of the combination of indirect competitive ELISA and the ICA using anti-BA MAb as a quality control method was confirmed for analysis of BA in Scutellariae Radix and Kampo medicines with a sufficient sensitivity (200 ng mL(-1) to 2 mu g mL(-1)), obtainable in an easy and timely manner. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:189 / 193
页数:5
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